In this study, the hydrolysis of pretreated sorghum stem and rice husk was investigated at various initial enzyme concentrations and substrate loadings. The slowdown in enzymatic hydrolysis of lignocellulosic materials with conversion has often been attributed to decreasing the activity of enzyme. A kinetic model was developed and expressed mathematically based on enzyme deactivation for enzyma...

pectin is used as gelatinizing agent in food industry. in this study pectin was extracted from five commercial sugar beet varieties namely: doroty, rasul, 7112, br1 and ic using pre-hydrolysis technique. experiments were conducted using factorial design to verify the effect of temperature, time, percentage of hcl and water requirements on the extraction rate of pectin. the qualitative factors o...

lipase from candida antarctica, fixed on macroporous acrylic resin, has been used for the intermediate production of mono- and diolein by hydrolysis of triolein. the effect of altering concentrations of triolein and glycerol and the function of the molecular sieve on the hydrolysis reaction of triolein were investigated. the highest hydrolysis yield was observed for the utmost concentration of ...

Abstract The aim of the present study was to determine changes in organic nitrogen fraction liquid digestate during laboratory tests mono- and co-digestion. Three agricultural wastes were tested: distillery residue, cattle slurry corn silage. In mono-fermentation tests, initial total concentration depended on feedstock used. As a result hydrolysis ammonification matter contained feedstocks, the...

An extracellular phytase from Aspergillus niger 11T53A9 was purified about 51-fold to apparent homogeneity with a recovery of 20.3% referred to the phytase activity in the crude extract. Purification was achieved by ammonium sulphate precipitation, ion chromataography and gel filtration. The purified enzyme behaved as a monomeric protein with a molecular mass of about 85 kDa and exhibited maxim...

The 4’-phosphopantetheine hyd.rolase of rat liver, partially purified by ammonium sulfate precipitation, catalyzes the hydrolysis of the prosthetic group 4’phosphopantetheine from the holo-fatty acid synthetase. The two products of the action of this enzyme, 4’phosphopantetheine and apo-fatty acid synthetase, were isolated by DEAE-cellulose chromatography and by chromatography on a Sepharose e-...

Urea is generated in humans following the breakdown of amino acids and is evenly distributed throughout the body, including in the central nervous system, subcutaneous adipose tissue, blood serum, and epithelial lining fluid [1,2]. Various pathogenic microbes are able to utilise urea as a nitrogen source through the activity of the enzyme urease that converts urea into ammonia and carbamic acid...

The aerobic biodegradability of the high explosive CL-20 by activated sludge and the white rot fungus Phanerochaete chrysosporium has been investigated. Although activated sludge is not effective in degrading CL-20 directly, it can mineralize the alkaline hydrolysis products. Phanerochaete chrysosporium degrades CL-20 in the presence of supplementary carbon and nitrogen sources. Biodegradation ...

Grafting of vinyl monomers onto cellulose is an important tool for the modification of cellulose. Depending on the monomer grafted onto cellulose, it gains new properties. The grafting can be performed in heterogeneous or homogeneous medium. In the grafting performed in heterogeneous medium, the reaction is carried out in aqueous medium using a suitable initiator. As initiator, the radiation or...