Elevated enzyme activities in plasma may at times be attributed to the presence of macro-enzymes. The macro-enzymes are often serum enzymes in complex with immunoglobulins, resulting in a greater molecular mass that cannot be filtered by renal glomeruli and are, hence, retained in the plasma. The aspartate aminotransferase (AST) can exist as a macro-enzyme, although it has been rarely reported....

The concentrations of pyridoxal 5'-phosphate, and the holoenzyme activities and apoenzyme contents of alanine aminotransferase and aspartate aminotransferase in plasma were determined simultaneously in healthy individuals, patients with renal insufficiency with and without chronic haemodialysis and in patients with acute myocardial infarction. Plasma pyridoxal 5'-phosphate is significantly dimi...

Muscle branched-chain amino acid metabolism is coupled to alanine formation via branched-chain amino acid aminotransferase and alanine aminotransferase, but the subcellular distributions of these and other associated enzymes are uncertain. Recovery of branched-chain aminotransferase in the cytosol fraction after differential centrifugation was shown to be accompanied by leakage of mitochondrial...

The activity of aspartate aminotransferase a vitamin B6 containing enzyme was measured in the laysed synaptosomes prepared from rat brain, during development. Administration of vitamin B6 increased the enzyme activity throughout development. The stimulating effect of this vitamin was prevented when the animals were previously treated with D-penicillamine. However, penicillamine alone had n...

  The aims of the present study were to determine the best time for egg stripping after ovulation and to study oocyte over-ripening in the Caspian brown trout (Salmo trutta caspius). Eggs were retained in the female abdominal cavity for 40 days post ovulation (DPO). Partial volumes of eggs stripped from 10 individually identified females at 10 days intervals and fertilized with a pool of semen ...

this study was conducted to determine the effect of dietary turmeric rhizome powder (trp) as a natural antioxidant, and soybean oil, canola oil and tallow on lipid metabolism, blood metabolites, immune system, and antioxidant status of broiler chickens before (bhs, 28 d) and after exposure to heat stress (ahs, 42 d). seven hundred and ninety two d-old male arian broilers were randomly allotted ...

The primary structure of tyrosine aminotransferase, as deduced from the nucleotide sequence of complementary DNA, was confirmed by fast atom bombardment mass spectrometry of tryptic peptides derived from the purified protein. Limited digestion of the native enzyme with trypsin released an acetylated, amino-terminal peptide; the new amino terminus in the modified enzyme was Val65. Endogenous pro...

conclusions office workers with chronic hepatitis c, treated with pegylated-interferon-α2b plus ribavirin, had work performance loss. in subjects treated with acetyl-l-carnitine supplementation we observed increased daily activity and reduced presenteeism and fatigue. acetyl-l-carnitinegroup had a smaller reduction of productivity comparing to placebo group. results significant difference were ...

the activity of aspartate aminotransferase a vitamin b6 containing enzyme was measured in the laysed synaptosomes prepared from rat brain, during development. administration of vitamin b6 increased the enzyme activity throughout development. the stimulating effect of this vitamin was prevented when the animals were previously treated with d-penicillamine. however, penicillamine alone had no eff...

By selective enrichment, we isolated a bacterium that can use β-phenylalanine as a sole nitrogen source. It was identified by 16S rRNA gene sequencing as a strain of Variovorax paradoxus. Enzyme assays revealed an aminotransferase activity. Partial genome sequencing and screening of a cosmid DNA library resulted in the identification of a 1,302-bp aminotransferase gene, which encodes a 46,416-D...