Acute renal failure is a common complication caused by Bothrops viper envenomation. In this study, the nefrotoxicity of a main component of B. leucurus venom called L-aminoacid oxidase (LAAO-Bl) was evaluated by using tubular epithelial cell lines MDCK and HK-2 and perfused kidney from rats. LAAO-Bl exhibited cytotoxicity, inducing apoptosis and necrosis in MDCK and HK-2 cell lines in a concent...

The objective of the present study was to identify a camptothecin (CPT) prodrug with optimal release and cytotoxicity properties for immobilization on a passively targeted microparticle delivery system. A series of R-amino acid ester prodrugs of CPT were synthesized, characterized, and evaluated. Four CPT prodrugs were synthesized with increasing aliphatic chain length (glycine (Gly) (2a), alan...

2 MD studies of the protein in micelles point to a lower degree of flexibility of huAS(A53T) with respect to the huAS(wt) (39). MD studies of the protein in lipid bilayer provide also interesting insights on the wild type structures, suggesting in particular that the truncated protein (residues 1–95) forms a bent helix due to its collective motions (40), and that the truncated fragment (residue...

In the past a good number of rotamer libraries have been published, which allow a deeper understanding of the conformational behavior of amino acid residues in proteins. Since the number of available high resolution X-ray protein structures has grown significantly over the last years, a more comprehensive analysis of the conformational behavior is possible today. In this paper, we present a met...

Calcium/aminoacids based MOFs (CaHis, CaPhe, and CaTrp) were synthesized by the hydrothermal method. Then, these materials applied as a filler in synthesis of composites hydrogel state on biopolymer matrix collagen -guar gum (CG). The calcium characterized ATR-FTIR XRD, while morphology, degradation profile, crosslinking swelling degree, proliferation cell viability porcine dermis fibroblasts c...

It has often been asserted that the discovery of the structure of DNA in 1953 was the starting point for molecular biology. Whether or not this is true, it cannot be doubted that Francis Crick became a dominant figure in the early years of molecular biology. In those years there was a continual interaction between theory and experiment, in which Crick paid a leading role. I was in Cambridge at ...