Amino acid supplementation has been shown to enhance the liver-specific functions of cultured hepatocytes during plasma exposure. However, their transport through the cell membrane may restrict their availability for hepatic metabolism. Here, we focus on transport constraints related to uptake of the neutral amino acids and their impact on hepatic metabolism and liver-specific functions. Under ...

Prochymosin is one of the most important aspartic proteinases used as a milk-clotting enzyme in cheese production. In the present investigation we report sequence of cDNA encoding goat ( Capra hircus ) preprochymosin and compare its nucleotide and deduced amino acid sequences with sequences of other ruminants preprochymosin. As bovine prochymosin, the caprine prochymosin cDNA encodes 365 amino ...

Sheikh K, Camejo G, Lanne B, Halvarsson T, Landergren MR, Oakes ND. Beyond lipids, pharmacological PPAR activation has important effects on amino acid metabolism as studied in the rat. Am J Physiol Endocrinol Metab 292: E1157–E1165, 2007. First published December 12, 2006; doi:10.1152/ajpendo.00254.2006.—PPAR agonists have been characterized largely in terms of their effects on lipids and gluco...

prochymosin is one of the most important aspartic proteinases used as a milk-clotting enzyme in cheese production. in the present investigation we report sequence of cdna encoding goat ( capra hircus ) preprochymosin and compare its nucleotide and deduced amino acid sequences with sequences of other ruminants preprochymosin. as bovine prochymosin, the caprine prochymosin cdna encodes 365 amino ...

Glucagon-like peptide-2 (GLP-2) increases small intestinal mass and blood flow in ruminant calves, but its impact on nutrient metabolism across the portal-drained viscera (PDV) and liver is unknown. Eight Holstein calves with catheters in the carotid artery, mesenteric vein, portal vein and hepatic vein were paired by age and randomly assigned to control (0.5% bovine serum albumin in saline; n ...

BACKGROUND Lactococcus lactis is recognised as a safe (GRAS) microorganism and has hence gained interest in numerous biotechnological approaches. As it is fastidious for several amino acids, optimization of processes which involve this organism requires a thorough understanding of its metabolic regulations during multisubstrate growth. RESULTS Using glucose limited continuous cultivations, sp...

Inborn errors of amino-acids metabolism and other inherited Mendeliandisorders are common in the MiddleEast.The number of diagnosed inborn errors of amino acid metabolism is growing constantly on account of and availability and improved of analytical techniques.  The aim of this work was to determine a rough estimate of the incidence rates of phenylketonuria (PKU), tyrosinemia, and maple syrup ...

This study was prompted by the previous finding of amino-aciduria (Choremis, Zannos, and Basti, 1957) and qualitative and quantitative changes of serum proteins in thalassaemia and sickle-cell anaemia (Allamanis, 1955; Afentaki, 1949; Minnich, Na-Nakorn, Chongchareonsuk, and Kochaseni, 1954). The electrophoretic pattern shows a fall in albumin, a moderate fall in the a, a29 and I globulin fract...

drogenase in normal human skin. In comparison with glutamate dehydrogenase, which catalyzes reversible oxidative deamination, the transaminases catalyze transfer of amino groups, usually from an amino acid to a ketoacid, without net loss of nitrogen. In other words, transamination represents the intermolecular exchange of amino nitrogen. Transamination plays a significant role in metabolism by ...