Ewa Seweryn3*, A rtur Pędyczakb and Teresa Banaś3 a Wroclaw Medical University, Department of Medical Biochemistry, Chalubiriskiego 10, 50-368 Wroclaw. Poland. Fax: (+48) 0713285415. E-mail: [email protected] b University of Wroclaw, Department of Chemistry, Wroclaw, Poland * Author for correspondence and reprint requests Z. Naturforsch. 55c, 657-660 (2000); received January 10/April 11, 20...

A protein of subunit Mr 34,000 (corrected here and subsequently to 36,000) has been isolated from a muscle homogenate which catalyzes the formation of the triad junction from isolated transverse tubules and terminal cisternae. This protein is identified as glyceraldehyde-3-phosphate dehydrogenase on the basis of N-terminal amino acid sequence, amino acid composition, and enzymic activity. The o...

The gene encoding Leifsonia alcohol dehydrogenase (LSADH), a useful biocatalyst for producing (R)-chiral alcohols, was cloned from the genomic DNA of Leifsonia sp. S749. The gene contained an opening reading frame consisting of 756 nucleotides corresponding to 251 amino acid residues. The subunit molecular weight was calculated to be 24,999, which was consistent with that determined by polyacry...

The amino acid sequence of porcine heart mitochondrial malate dehydrogenase (mMDH; L-malate: NAD+ oxidoreductase, EC 1.1.1.37) has been compared with the sequences of six different lactate dehydrogenases (LDH; L-lactate: NAD+ oxidoreductase, EC 1.1.1.27) and with the "x-ray" sequence of cytoplasmic malate dehydrogenase (sMDH). The main points are that (i) all three enzymes are homologous; (ii) ...

Abstract The aim of the present study is to explore new selective anti-inflammatory compounds with low cardiovascular risk. Twelve thiadiazole derivatives incorporating different amino acid moieties were newly synthesized ( 4 – 15 ) as potential agents risks through dual COX-2/MPO inhibition. Compounds initially screened for their effect by assay COX-2, most potent 6 , 8 further tested COX-1 in...

As part of a larger project dealing with an in vestigation of amino acid and protein metabolism in leukemia, a study was undertaken to determine those enzymes concerned with amino acid anabo lism and catabolism as they occur in selected tis sues. Several review papers (@,4, 11, 1@)have ade quately summarized our present knowledge of the metabolism of white blood cells (WBC) in leuke mia. Howeve...

Isolated membrane vesicles from Escherichia coli B grown on DL-alanine and glycerol carry out amino acid active transport coupled to a membrane-bound D-alanine dehydrogenase (Kaczorowski, G., Shaw, L., Fuentes, M., and Walsh, C. (1975) J. Biol. Chem. 250, 2855). Certain L-amino acids can also energize solute transport by conversion to their D isomers via an alanine reacemase. Both D-chloroalani...

-1. Amino acid, keto acid and imino acid substrate specificities of octopine dehydrogenase (ODH) from seven marine invertebrate sources were investigated. 2. Three groups of ODH enzymes were identified determined, largely, by their use of L-lysine as an alternative amino acid substrate, 3. The broadly specific ODH from the sea anemone, Calliactes parasitica, utilized L-arginine and L-lysine at ...