Abstract Amine transaminases (ATAs) are biocatalysts for the synthesis of chiral amines and can be identified in sequence databases by specific motifs. This study shows that activity level towards model substrate 1‐phenylethylamine predicted solely from sequence. To demonstrate this, 15 putative ATAs with a different distribution hydrophobic or hydrophilic amino acid side chains near active sit...

The catalytic space of the P450 monooxygenase CYP153AM.aq was opened from a terminal (ω-) fatty acid hydroxylase to catalyst capable performing ω-hydroxylation dodecylamine, which is potent inhibitor for wild-type enzyme. A simple screening method named Rapid-flow Analysis Product Peaks (RAPP) established and applied measure saturation libraries directly 96-deepwell plate in 36 seconds per samp...