The plant-specific Rop subfamily of Rho GTPases, most closely related to the mammalian Cdc42 and Rac GTPases, plays an important role in the regulation of calcium-dependent pollen tube growth, H(2)O(2)-mediated cell death, and many other processes in plants. In a search for Rop interactors using the two-hybrid method, we identified a family of Rho GTPase-activating proteins (GAP) from Arabidops...

Aluminofluoride and beryllofluoride complexes can activate the heterotrimeric G-proteins by binding next to GDP in the nucleotide site of their G alpha subunit and acting as analogs of the gamma-phosphate of a GTP. However, the exact structures of the activatory complexes in solution as well as those of the bound complexes in the nucleotide site are still disputed. We have studied, by monitorin...

Activation of a heterotrimeric G-protein by an agonist-stimulated G-protein-coupled receptor requires the propagation of structural signals from the receptor binding interface to the guanine nucleotide binding pocket of the G-protein. To probe the molecular basis of this signaling process, we are applying high resolution NMR to track structural changes in an isotope-labeled, full-length G-prote...

In this study, kenaf, a fast-growing plant, was pyrolyzed to biochar, and the biochar impregnated with aluminum improve its fluoride adsorption capacity. The Al-impregnated kenaf (Al-KNF-BC) at temperatures of 300–700 °C, where specimen treated 300 °C (Al-KNF-300) demonstrated highest kinetics equilibrium by Al-KNF-300 followed pseudo-second-order Langmuir models, respectively. According model,...

Within the energetic materials and additive manufacturing (AM) communities, a number of aluminum/fluoropolymer (Al/FP) combinations have been identified for their suitability in various techniques. For practical applications, such as case reactive wire or core solid propellant, range selectable reactivity within given Al/FP selection is needed. The purpose this study was to alter aluminum/polyv...