Azospirillum brasilense glutamate synthase (GltS) is the prototype of bacterial NADPH-dependent enzymes, a class of complex iron-sulfur flavoproteins essential in ammonia assimilation processes. The catalytically active GltS alpha beta holoenzyme and its isolated alpha and beta subunits (162 and 52 kDa, respectively) were analyzed using synchrotron radiation x-ray solution scattering. The GltS ...

High-resolution ac calorimetry has been used to study the nematic to smectic- A (N-SmA) phase transition in the liquid crystal octylcyanobiphenyl (8CB) confined in aligned colloidal aerosil gels. A stable and robust nematic alignment was achieved by repeated thermal cycling of the samples in the presence of a strong uniform magnetic field. In some ways (such as transition temperature and integr...

Cells react differently to clustered and dispersed DNA double strand breaks (DSB). Little is known about the initial reaction to simultaneous induction of DSBs with different complexities. Here, we used live cell microscopy to analyse the behaviour of 53BP1-GFP (green fluorescence protein) foci formation at DSBs induced in U2OS cells by alpha particles, X-rays or mixed beams over a 75 min perio...

We propose a fully automatic algorithm for view interpolation of a completely non-rigid dynamic event across both space and time. The algorithm operates by combining images captured across space to compute voxel models of the scene shape at each time instant, and images captured across time to compute the “scene flow” between the voxel models. The scene-flow is the non-rigid 3D motion of every ...

Background: CdWO4 is a scintillator with some unique properties. For example, high density, thermal and chemical stability and so on. Different applications of this scintillator such as X-ray scintillator has been investigated thoroughly so far. However, there is limited number of studies reporting the characteristics of CdWO4 as an alpha counter. Materials and Methods: The CdWO4 powder was syn...

Optical potential parameters in nuclear model calculations are determined by fitting elastic scattering angular distribution data. Due to the dominance of Coulomb part, is performed at much higher energies. As a result, change required for low-energy astrophysical reactions. A different approach suggested using ( $$\alpha $$ ,n) reaction determine modified alpha optical potentials suitable astr...

Simple pseudo-3D modifications to the constant-time HSQC and HCACO experiments are described that allow accurate (+/- 0.5 Hz) measurement of one bond JC alpha H alpha coupling constants in proteins that are uniformly enriched with 13C. An empirical phi,psi-surface is calculated which describes the deviation of 1JC alpha H alpha from its random coil value, using 203 1JC alpha H alpha values meas...