A method is presented for the calculation of REDOR dephasing for specifically labeled membrane-spanning peptides in uniformly aligned lipid bilayers under magic angle oriented sample spinning (MAOSS) conditions. Numerical simulations are performed for dephasing of (13)C signal by (15)N when the labels are placed in an alpha-helical peptide at the carbonyl of residue (i) and amide nitrogen of re...

BACKGROUND The main cause for revision hip arthroscopy surgery is incomplete bony resection of femoroacetabular impingement (FAI). This study aimed to compare the cam resection accuracy via the conventional hip arthroscopy technique with the navigation technique. METHODS Two prospectively randomized groups were recruited: navigated (n = 15) and conventional (n = 14). A pre-operative CT and po...

MOTIVATION Most secondary structure prediction programs target only alpha helix and beta sheet structures and summarize all other structures in the random coil pseudo class. However, such an assignment often ignores existing local ordering in so-called random coil regions. Signatures for such ordering are distinct dihedral angle pattern. For this reason, we propose as an alternative approach to...

We consider theoretically liquid rise against gravity in capillaries with height-dependent cross-sections. For a conical capillary made from a hydrophobic surface and dipped in a liquid reservoir, the equilibrium liquid height depends on the cone-opening angle alpha, the Young-Dupré contact angle theta, the cone radius at the reservoir's level R(0), and the capillary length kappa(-)(1). As alph...

The structure of 14-protofilament microtubules reassembled from dogfish shark brain tubulin was analyzed by high resolution electron microscopy and optical diffraction. The simultaneous imaging of the protofilaments from near and far sides of these tubules produces a moiré pattern with a period of approximately 96 nm. Optical diffraction patterns show that the 5-nm spots that arise from the pro...

Simple criteria, based on the combined use of nmr spectral parameters and potential energy maps, are proposed for the conformational analysis of polypeptides and proteins. Experimentally determined coupling constants (3)J(NC) for the N-C(alpha) bond are consistent with the Karplus-Bystrov relationship. It is proposed therefore that (3)J(NC) can be used to distinguish (a) between right-and left-...

We examined the 204-nm UV Raman spectra of the peptide XAO, which was previously found by Shi et al.'s NMR study to occur in aqueous solution in a polyproline II (PPII) conformation. The UV Raman spectra of XAO are essentially identical to the spectra of small peptides such as ala(5) and to the large 21-residue predominantly Ala peptide, AP. We conclude that the non-alpha-helical conformations ...

Theory,experimental aspects, and use in structure calculation of cross-correlated relaxation rates measured on zero- and double-quantum coherences in liquid state NMR are presented. The relative size of the interaction depends on the projection angle between the two tensorial interactions. The tensorial interaction can be either a dipolar interaction or a chemical shift anisotropy relaxation me...

Abstract Fix an irrational number $$\alpha $$ α , and consider a random walk on the circle in which at each step one moves to $$x+\alpha x + or $$x-\alpha - with probabilities 1/2, 1/2 provided current position is x . If observable give...