Real time monitoring of electrolyte resistance changes during hydrolysis of 4-nitrophenylphosphate (pNPP) by alkaline phosphatase (ALP) bound on paramagnetic-beads was performed into a small dielectric channel. The reaction kinetic fit with a non-competitive substrate-inhibition equation. Michaelis-Menten apparent constant, KM(app), was determined as 0.33±0.06mM and the maximum apparent rate, V...

INTRODUCTION AND OBJECTIVE Mandible is used as a bone model for monitoring bone tissue responses to various factors influencing skeletal homeostasis. Considering the lack of experimental data on interrelationships between bone metabolism indices and morphometric, densitometric and mechanical properties of mandible, the aim of this study was to perform such an evaluation in 6-month-old pigs. M...

Intestinal origin alkaline phosphatase activity (ALP) in plasma was measured by a sensitive immunocapture assay in 104 jaundiced patients--84 with intrahepatic and 20 with post-hepatic jaundice. Increased enzyme activities were observed in those with intrahepatic disease and subnormal values in those with post-hepatic disease. At a discriminant level intestinal origin ALP showed a diagnostic se...

The influence of anaemia on alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) activities in dogs infected with B. canis was investigated. Samples of blood and serum from 230 infected dogs were divided into two groups: A (with anaemia) and B (without anaemia). The differences in the activities of the enzymes between both groups were not statisticall...

Case Summary: A 33-year old female with a history of Crohn’s disease presented with complaints of upper abdominal pain and bilious vomiting. She had been receiving Adalimumab and Mesalamine for treatment of Crohn’s disease. A liver panel revealed alkaline phosphatase (ALP): 188 IU/L, aspartate aminotransferase (AST): 1,638 IU/L, alanine transaminase (ALT): 592 IU/L, total bilirubin: 2.5 mg/dL, ...

The effects of six particulate metals (Al, Ti, Zr, Nb, Ta and Cr) on cell viability and alkaline phosphatase (ALP) activity were studied in vitro using two types of osteoblast-like cells, MG-63 and HOS cells. The cell viability in the presence of Al, Ti and Zr was depressed at lower concentrations than in the presence of Nb, Ta and Cr. The average sizes of the Al, Ti, Zr, Nb, Ta and Cr particul...

Metabolism of the bone healing process can be monitored by Bone formation marker, such as alkaline phosphate (ALP). Simvastatin is known to increase marker in repair phase, but its effects on ALP level have not been yet. This study aimed determine effect oral simvastatin administration expression femur fracture a rat model fracture. The research method was experimental post-test only using 18 R...

Pestisida adalah bahan kimia yang digunakan untuk meracuni hama. Petani di Indonesia banyak menggunakan pestisida namun pula tidak memahami aturan penggunaan nya. Semakin sering petani terpapar maka akan menimbulkan dampak kesehatan yaitu gangguan fungsi hati, salah satu parameter pemeriksaan hati enzim Alkaline Phosphatase (ALP). Enzim ALP diproduksi oleh sel dan pembentuk tulang. Penelitian i...

This study was designed to investigate the effects of molluscicidal components of Myristica fragrans Houtt. (Myristicaceae) on certain enzymes in the nervous tissue of freshwater snail Lymnaea acuminata Lamarck (Lymnaeidae). In vivo and in vitro treatments of trimyristin and myristicin (active molluscicidal components of Myristica fragrans Houtt.) significantly inhibited the acetylcholinesteras...