A standard assay for measuring alkaline phosphatase activity is detecting the production of nitrophenol from the artificial substrate p-nitrophenol phosphate. Nitrophenol absorbs light at 420 nm, which provides a convenient detection method. However, the absorbance of nitrophenol varies with pH. This variation complicates the use of the colorimetric assay to study the pH-dependence of alkaline ...

The interactions of Mg 2+ and Zn 2+ ions in the activation of non-specific tissue alkaline phosphatase were investigated using crude extracts of rat kidney. Activation of alkaline phosphatase by the metal ions was accompanied by changes in the kinetic parameters of pnitrophenylphosphate hydrolysis. The results suggest some synergistic interactions between Mg 2+ and Zn 2+ ions in promoting the h...

The aim of this study was to determine the effect of mineral fertilization on the activity of alkaline and acid phosphatase and content of available phosphorus in soil under winter wheat cultivation. The fi rst factor of experiment was the fertilization with P, K, Mg, Ca and S in six levels: 1 (P, K, Mg, Ca and S), 2 (K, Mg, Ca and S), 3 (P, Mg, Ca and S), 4 (P, K, Ca and S), 5 (P, K, Mg, Ca), ...

Human milk is the main source of nutrients for a child during the first months of life. Alkaline phosphatase (EC 3.1.3.1) is a very important enzyme in clinical chemistry because of its activity in various tissues and biological fluids, being an indicator of physiological or diseased states. Milk contains several phosphatases, the principal ones being alkaline and acid phosphomonoesterases whic...

A correlation was made between the urinary excretion of GOT, GPT, LDH, HBD, and acid and alkaline phosphatase and their serum activity in 18 suspected cases of myocardial infarction. The diagnoses were made by a cardiologist without knowledge of the enzyme assays. The study included 2 cases of nontransmural infarction; 5 cases that had not sustained an infarction served as controls. Elevations ...

an experiment was conducted to evaluate the effect of replacing dietary limestone with mashhad power plant calcium-waste in combination with available phosphorus level on growth performance, tibial strength and blood alkaline phosphatase activity in broiler chickens. three hundred and eighty four male broiler chickens were used in a complete randomized design experiment with a 4×2 factorial arr...

this study focused on use of bacterial extracellular enzyme activities as biochemical parameters, along with the microbiological and physicochemical characteristics, in a comprehensive assessment of water quality of four lake ecosystems in the province of vojvodina (northern serbia): provala, ludas, zobnatica and palic. water samples were collected in june and october, 2008. for assessment of ...

The roles of alkaline phosphatase and labile internal mineral in matrix vesicle-mediated mineralization have been studied by selectively releasing the enzyme from a wide variety of matrix vesicle preparations using treatment with a bacterial phosphatidylinositolspecific phospholipase C and by demineralization of the vesicles using isosmotic pH 6 buffer. Following depletion of 50-9070 of the alk...

CYP3A4 probably has the broadest catalytic activity of any cytochrome P450. It is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. Firstly, a total of 300 bp core distal enhancer of CYP3A4 XREM region (-7972/-7673) were amplified from human genomic DNA. The PCR product was then ligated into a human secretory alkaline phosphat...

35 epileptic patients, aged 10 to 58 years (mean 23), who were taking anticonvulsant drugs were studied. The patients exhibited a 34% reduction in serum calcium levels, a 41 % increase in serum alkaline phosphatase activity and a slight but insignificant decrease in serum phosphate, compared to untreated controls. These changes appear to be related to the anticonvulsant drug taken, in the ...