OBJECTIVE To determine whether serum vitamin D levels are correlated with serum levels of alkaline phosphatase or not. STUDY DESIGN Cross-sectional, observational study. PLACE AND DURATION OF STUDY Multi-centre study, conducted at Liaquat National Hospital and Medical College, National Medical Centre and Medicare Hospital, Karachi, from January to October 2009. METHODOLOGY Patients attend...

Following pulmonary infection with Francisella tularensis, we observed an unexpected but significant reduction of alkaline phosphatase, an enzyme normally up-regulated following inflammation. However, no reduction was observed in mice infected with a closely related gram-negative pneumonic organism (Klebsiella pneumoniae) suggesting the inhibition may be Francisella-specific. In similar fashion...

uridine (lUdR) there was an increase in alkaline phosphatase activity after about 24 hr, reaching a maximum usually at about 72 hi. At this concentration, during this time, there was a relatively small inhibition of cell replication. The increment in alkaline phosphatase activity was in large measure prevented by concomitant addition of 10 /¿Mthymidine. Further, lUdR did not increase alkaline ...

Ewen, Lilian M.: Separation of alkaline phosphatase isoenzymes and evaluation of the clinical usefulness of this determination. Am. J. Clin. Pathol. 61: 142-154, 1974. A simple technic for separation of alkaline phosphatase isoenzymes on agarose gel, using thymolphthalein monophosphate as the isoenzyme indicator substrate, is described. Results demonstrate that separation of isoenzymes of alkal...

Alkaline phosphatase serves both as a model enzyme for studies on the mechanism and kinetics of phosphomonoesterases and as a reporter in enzyme-linked immunosorbent assays (ELISAs) and other biochemical methods. The tight binding of the enzyme to its inorganic phosphate product leads to strong inhibition of catalysis and confounds measurements of alkaline phosphatase activity. We have develope...

The present study has been carried on changes in activity of alkaline phosphatase in serum and gastrocnemius muscle of mice after sub-chronic use of diclofenac. Mice in experimental group received diclofenac (10 mg/kg body wt /day) for 30 days while control group received normal saline. Alkaline phosphatase was assayed in muscle and serum and its activity was localized histochemically in muscle...

The influence of non-steroidal anti-inflammatory drugs (NSAIDs) and of disease activity on the serum alkaline phosphatase concentration was examined in patients with rheumatoid arthritis, osteoarthritis, and polymyalgia rheumatica. Concentrations of serum alkaline phosphatase were similar both in patients with rheumatoid arthritis taking NSAIDs and in those not taking NSAIDs. In patients with o...

It is generally recognized that a dependable test of placental function is one of the most urgent needs in obstetrics. The advantages of using various placental enz,ymes as an index of placental function were found in the fact that their measurements are simple procedures. Coryn (1934) showed for the first time that serum alkaline phosphatase is elevated in late pregnancy. Serum alkaline phosph...

BACKGROUND This study was performed in an attempt to assess serum calcium, phosphorus, and alkaline phosphatase in adolescent girls and calcium supplementation in subjects with abnormal serum findings. METHODS A randomized, cross-sectional, cluster sampling, prospective, and descriptive trial was conducted from January through March 2003 for measurement of serum calcium, phosphorus, and alkal...