Rats were vaccinated with a toxoid (D-toxoid) prepared from purified heat-labile toxin (D-toxin) produced by Pasteurella multocida serogroup D. Vaccination of rats with D-toxoid prevented death and other effects of D-toxin (hepatic necrosis, development of elevated leukocyte counts, lymphopenia, neutrophilia, and elevated complement titers) that occurred in phosphate buffered saline (PBS)-vacci...

OBJECTIVES The objective of this study was to investigate the effect of a laminin coating on calcium phosphate precipitation on three potentially bioactive titanium surfaces in simulated body fluid. MATERIAL AND METHODS Blasted titanium discs were prepared by alkali and heat treatment (AH), anodic oxidation (AO) or hydroxyapatite coating (HA) and subsequently coated with laminin. A laminin co...

esis. One is to trace step by step the process of oncogenic transformation in cells assembled from components, DNA, chromatin, nuclei, and cytoplasm, that have been treated with carcinogens under carefully controlled conditions in vitro ; the other approach is to establish uniform associa tions between the process of oncogenic transformation and other processes that are better understood at the...

An Investigation of the Role of Microsomal Oxidative Metabolism in the in Vivo Genotoxicity of 1,2-Dichloroethane. STORER, R. D.. AND CONOLLY, R. B. (1985). Toxicol. Appl. Pharmacol. 77,36-46. In vitro studies have demonstrated that two different metabolic pathways, glutathione conjugation mediated by the glutathione S-transferases and microsomal oxidation, may be involved in the genotoxicity a...

Recent studies have demonstrated the genotoxicity of anesthetics in patients who have undergone surgery and in personnel who are occupationally exposed to anesthetics. However, these findings are controversial. Herein, we used the comet assay (single-cell gel electrophoresis) to investigate the genotoxic effects of two volatile compounds [isoflurane (ISF) and sevoflurane (SVF)] that are used in...

Alterations in checkpoint and DNA repair pathways may provide adaptive mechanisms contributing to acquired drug resistance. Here, we investigated the levels of proteins mediating DNA damage signaling and -repair in RPMI8226 multiple myeloma cells and its Melphalan-resistant derivative 8226-LR5. We observed markedly reduced steady-state levels of DNA glycosylases UNG2, NEIL1 and MPG in the resis...

1. Gramicidin caused H(+) production by liver mitochondria; this process was dependent on the presence of Na(+), Li(+), Rb(+) or Cs(+). In the presence of one of these alkali-metal ions and phosphate, gramicidin caused mitochondrial swelling and increased oxygen consumption. 2. Uncoupling agents, anaerobic conditions or respiratory inhibitors both inhibited and reversed H(+) production and swel...