The spectrophotometric determination of Cu(II) with an anthraquinone derivative (Alizarin Red S) has been investigated. The experimental conditions, such as the pH of the sample and concentration of ligand, were optimized. This method is simple and sensitive for determination of Cu(II) ion. The interfering effects of diverse ions were investigated. Copper ion was determined by measuring the abs...

We investigated the effects of Ga-Al-As laser irradiation on the mineralization ability of human dental pulp (HDP) cells and on Smads and bone morphogenetic protein (BMP) production as one mechanism for the transmission of laser photochemical energy to cells. HDP cells in vitro were irradiated once with a Ga-Al-As laser at 1.0 W for 500 s, and calcified nodule formation was assessed by Alizarin...

This study was designed to assess the effects of cold atmospheric plasma on osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. Plasma was irradiated directly to a culture medium containing plated cells for 5 s or 10 s. Alkaline phosphatase (ALP) activity assay and alizarin red staining were applied to assess osteoblastic differentiation. The plasma-generated radicals were detected...

STATEMENT OF THE PROBLEM Stem cells from human exfoliated deciduous teeth (SHEDs) are a population of highly proliferative cells, being capable of differentiating into osteogenic, odontogenic, adipocytes, and neural cells. Vitamin D3 metabolites such as 1α, 25-dihydroxyvitamin D3 are key factors in the regulation of bone metabolism. PURPOSE The aim of this study was to investigate the effect ...

Two fluorescent dyes, alizarin red S (ARS) and calcein (CAL), were applied to evaluate the marking effects on juveniles of Acanthopagrus schlegelii. The total mortality rates experimental groups significantly lower (p < 0.05) than those control groups, but no significant difference was detected between two staining methods. fluorescence microscopy observation results showed that quality ARS ...