510 Conclusions We have shown evidence for the bridging flocculation of PSL by p-LG. The change in surface charge of the PSL spheres resulting from adsorption of p-LG molecules correlates with the formation of aggregates bridged by protein molecules. To develop models of the kinetics of bridging flocculation, some measurements of aggregation as a function of time and temperature have been carri...

Corn starch gels (1:4 w:w) were made with the addition (up to 2% w/w basis) of sodium alginates different average molecular sizes and nature. Rheology starchy characterized evaluate their viscoelasticity. Gels subjected in vitro digestion estimate effect on glycemic response. Tested significantly decreased value elastic modulus gels. The coordination number obtained from complex indicated that ...

Sodium polyacrylate (PANa) is a water-soluble, high-molecular compound, and its aqueous solution shows a very high viscosity and stringiness. In the present study, preventive effects of PANa on three kinds of esophageal lesions induced by gastric juice were examined in comparison with those of aceglutamide aluminum and sodium alginate. The influences of PANa on gastric contents were also studie...

Endo-type alginate lyases usually degrade alginate completely into various size-defined unsaturated oligosaccharide products (≥disaccharides), while exoenzymes primarily produce monosaccharide products including saturated mannuronate (M) and guluronate (G) units and particularly unsaturated Δ units. Recently, two bifunctional alginate lyases have been identified as endolytic but M- and G-produc...

The modification of bioresorbable polyester surfaces in order to alter their biointeractions presents an important problem in biomedical polymer science. In this study, the covalent modification of the surface of poly(lactic acid)-based (PLA-based) films with poly(acryl amide) and sodium alginate hydrogels was performed to change the non-specific polyester interaction with proteins and cells, a...

Alginate production by the highly alginate-producing Pseudomonas aeruginosa 8821M was maximal at a dissolved oxygen tension (DOT) of 5% of air saturation. Lower DOT limited growth and alginate synthesis. At higher DOT values up to 70% of air saturation, the specific alginate production rate decreased. Nevertheless, the molecular mass of the alginate increased at higher aerations, as indicated b...

Alginate microspheres containing furosemide were prepared by the ionotropic external gelation technique using Ca2+, Al3+ and Ba2+ ions. The incorporation efficiency of the prepared microspheres ranged between 65% and 93%. The effect of sodium alginate concentration, cross-linking ions and drying conditions was evaluated with respect to entrapment efficiency, particle size, surface characteristi...

Alginate-based microcapsules are used for immunoisolation of cells to release therapeutics on a minute-to-minute basis. Unfortunately, alginate-based microcapsules are suffering from varying degrees of success, which is usually attributed to differences in tissue responses. This results in failure of the therapeutic cells. In the present study we show that commercial, crude alginates may contai...

Alginate is known to prevent elimination of Pseudomonas aeruginosa biofilms. Alginate lyase (AlgL) might therefore facilitate treatment of Pseudomonas aeruginosa-infected cystic fibrosis patients. However, the catalytic activity of wild-type AlgL is not sufficiently high. Therefore, molecular modeling and site-directed mutagenesis of AlgL might assist in enzyme engineering for therapeutic devel...

In this study, the synthesis of fatty acid – carbohydrate esters used glucose (G) and coconut oil fatty acid (FA). The esterification reaction was carried out enzymatically using Candida rugosalipase in organic solvent (n-hexane). Lipase was immobilized on Ca-alginate gel beads withthe optimum condition for immobilization was1 % of Na-alginate, resulting 61.66% immobilization yield. The optimum...