نتایج جستجو برای: alamar blue test
تعداد نتایج: 877687 فیلتر نتایج به سال:
Carbon nanotubes can be either single-walled or multi-walled, each of which is known to have a different electron arrangement and as a result have different properties. However, the shared unique properties of both types of carbon nanotubes (CNT) allow for their potential use in various biomedical devices and therapies. Some of the most common properties of these materials include the ability t...
Objectives: The genus Salvia is one of the most widespread members of the Lamiaceae family and S.virgata Jacq. decoction is traditionally used for leukemia in Turkey. In this study, water extract and methanol extract of S. virgata were investigated for their antiproliferative effects on Jurkat (Clone E6-1) leukemia cell lines and also GSH-Px, SOD and MDA levels were evaluated as oxidative stres...
The combination of traditional herbal medicine (phytotherapeutic agents) with bioactive glasses is a promising strategy to generate advanced scaffolds for bone tissue engineering (BTE). An old remedy used wound care since ancient times honey. antioxidant, antimicrobial and antibacterial properties Manuka honey, in particular, make it an attractive substance application BTE prevent infections bi...
OBJECTIVES To evaluate existing protocols, based on Alamar blue (resazurin), for the routine determination of drug susceptibility in trichomonads, develop new ones and validate these by screening small antiprotozoal libraries. METHODS The resazurin-based assay was evaluated by determining fluorescence development in Trichomonas media with various cell densities after various intervals and in ...
The single agent of amrubicin is active in untreated small-cell lung cancer (SCLC). Cytotoxicity of amrubicinol, the active form of amrubicin, was evaluated in a parent SCLC cell line (SBC-3); an active metabolite of irinotecan, 7-ethyl-10-hydroxy-camptothecin (SN-38)-resistant subline (SBC-3/SN-38); and cisplatin-resistant subline (SBC-3/CDDP) using AlamarBlue assay. Interaction of the combine...
Anthrax toxin consists of protective antigen (PA) and two toxic components, lethal factor (LF) and edema factor (EF). PA binds to mammalian cellular receptors and delivers the toxic components to the cytoplasm. PA is the primary antigenic component of the current anthrax vaccine. Immunity is due to the generation of antibodies that prevent the PA-mediated internalization of LF and EF. In this s...
The ability of two types of single walled carbon nanotubes (SWCNT), namely Arc Discharge (AD) and HiPco single walled carbon nanotubes, to induce an indirect cytotoxicity in A549 lung cells by means of medium depletion was investigated. The nanotubes were dispersed in a commercial cell culture medium and subsequently removed by centrifugation and filtration. Spectroscopic analysis confirmed the...
Human immune response is compromised and bacteria can become more antibiotic resistant in space microgravity (MG). We report that under low-shear modeled microgravity (LSMMG), stationary-phase uropathogenic Escherichia coli (UPEC) become more resistant to gentamicin (Gm), and that this increase is dependent on the presence of σs (a transcription regulator encoded by the rpoS gene). UPEC causes ...
Due to the importance of neural stem cells (NSCs) in plasticity of the nervous system and treating neurodegenerative diseases, the main goal of this study was to evaluate the effects of radiofrequency radiation emitted from a GSM 900-MHz mobile phone with different exposure duration on proliferation, differentiation and apoptosis of adult murine NSCs in vitro. We used neurosphere assay to evalu...
We have developed and optimized a 96-well microtiter plate assay, based on the reduction of alamarBlue, to assess the efficacies of much needed new antimicrobials against Acanthamoeba species. This assay has been optimized for determination of drug efficacy against two potentially pathogenic species, Acanthamoeba castellanii and Acanthamoeba polyphaga, and has been validated by comparison of th...
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