Using the tip-growing pollen tube of Arabidopsis thaliana and Nicotiana tabacum as a model to investigate endocytosis mechanisms, we show that phosphatidylinositol-4-phosphate 5-kinase 6 (PIP5K6) regulates clathrin-dependent endocytosis in pollen tubes. Green fluorescent protein-tagged PIP5K6 was preferentially localized to the subapical plasma membrane (PM) in pollen tubes where it apparently ...

Sac1 phosphoinositide (PI) phosphatases are essential regulators of PI-signaling networks. Yeast Sac1, an integral endoplasmic reticulum (ER) membrane protein, controls PI4P levels at the ER, Golgi, and plasma membrane (PM). Whether Sac1 can act in trans and turn over PI4P at the Golgi and PM from the ER remains a paradox. We find that Sac1-mediated PI4P metabolism requires the oxysterol-bindin...

This paper deals with an integrated model for the joint determination of economic production quantity and preventive maintenance (PM) level for an imperfect process having a general deterioration distribution with increasing hazard rate. The effect of PM activities on the deterioration pattern of the process is modeled using the imperfect maintenance concept. It is assumed that, after each PM, ...

Young (ages 18-22 years) and older (ages 61-87 years) adults (N = 106) played the Virtual Week board game, which involves simulating common prospective memory (PM) tasks of everyday life (e.g., taking medication), and performed working memory (WM) and vigilance tasks. The Virtual Week game includes regular (repeated) and irregular (nonrepeated) PM tasks with cues that are either more or less fo...

BACKGROUND Exposure to ambient air particulate matter (PM) has been shown to increase rates of cardiopulmonary morbidity and mortality, but the underlying mechanisms are still not well understood. OBJECTIVE We examined signaling events involved in the expression of the inflammatory gene interleukin-8 (IL-8) in human airway epithelial cells (HAECs) exposed to ambient PM collected in an urban a...

Nanometer-spaced appositions between endoplasmic reticulum and plasma membrane (ER-PM junctions) stabilized by membrane-joining protein complexes are critically involved in cellular Ca2+-handling and lipid trafficking. ER-PM junctional architecture and plasticity associated with inter-membrane communication are as yet barely understood. Here, we introduce a method to precisely characterize ER-P...

9 The objectives of this study were to estimate variance components, genetic 10 parameters, EBV, accuracies, and rankings for nine ultrasound and carcass traits in a 11 multibreed Angus-Brahman population using three genomic-polygenic models and one 12 polygenic model (PM). The genomic-polygenic models used the complete GeneSeek 13 GPF250k SNP set (GPM), top 5% SNP (GPMR1), and 5% SNP evenly sp...

We observed a panel of 133 children (5-13 years of age) with asthma residing in the greater Seattle, Washington, area for an average of 58 days (range 28-112 days) during screening for enrollment in the Childhood Asthma Management Program (CAMP) study. Daily self-reports of asthma symptoms were obtained from study diaries and compared with ambient air pollution levels in marginal repeated measu...

We present the first large-scale comparison of prospective memory (PM) and retrospective memory (RM) from 8 to 50 years of age (N = 318,614). Participants in an Internet study were asked to remember to click on a smiley face (single-trial event-based PM test) and to indicate whether/where a picture had changed from study to test (single-trial RM test), in both cases after retention intervals fi...