Plate I Photomicrographs of the human tooth germ. a. At the cap stage of development. The black punctate material is glycogen. b. At the bell stage of development. c. Undecalcified section illustrating the marked Schiff positivity of the odontoblast cytoplasm during dentinogenesis. d. Undecalcified section illustrating the distribution of alkaline phosphatase. Note the intense enzyme activity i...

Chemomechanical caries removal involves the chemical softening of carious dentine followed by its removal by gentle excavation. The reagent involved is generated by mixing amino acids with sodium hypochlorite; N-monochloroamino acids are formed which selectively degrade demineralised collagen in carious dentine. The procedure requires 5-15 minutes but avoids the painful removal of sound dentine...

BACKGROUND There are a number of studies citing the primary reason for replacing auto cure glass ionomer cements was due to recurrent caries. The purpose of this study was to use an in vitro model to measure caries at the dentine restoration interface of bonded composite resin and auto cure glass ionomer cement restorations and to measure the amount of surface degradation occurring in the resto...

Aim To compare the shear bond strength and microleakage of four adhesive systems to the enamel and dentine of primary bovine teeth. Methods 120 bovine primary mandibular incisors were collected and stored in an aqueous 1% chloramine solution at room temperature for no longer than 3 months after extraction (80 for shear bond testing and 40 for microleakage evaluation). The adhesives tested were ...

Laser scanning confocal microscopy (LSCM) coupled to a constant volume flow-pressure measuring system is introduced as a new technique for the quantitative measurement of fluid flow across porous materials. Such processes are ubiquitous from the life sciences to materials science and the methodology herein could find widespread application. The methodology has been applied to the detection of f...

This study was carried out to determine the effects of nerve resection and cavity preparation with restoration (CPR) on the formation of response dentine in continually developing rabbit incisor teeth. Resections of the inferior alveolar nerve (IAN) and/or the superior cervical ganglion (SCG) were performed on the right side of 42 adult New Zealand rabbits. Fifteen days were allowed for neural ...

OBJECTIVE To analyze possible correlations among tubular dentine cement penetration, adhesiveness and apical leakage in fillings performed with gutta percha and an endodontic cement based on epoxy amine resin. MATERIAL AND METHODS Sixty similar, extracted human mandibular central incisors were irrigated, instrumented and filled following the same protocol. First, apical leakage was quantified...

BACKGROUND Adhesive systems are constantly subjected to mechanical and chemical stresses that negatively impact the integrity and durability of the dentine-adhesive interface. Despite the lack of evidence to support or reject the clinical indication for mechanical retention, the potential further contribution of these preparations to the behavior of the composite resin-sound dentine bond has be...

OBJECTIVES Search in PubMed with keywords "enzymes, dentinogenesis, and dental caries" revealed only 4 items, but when combined with "enzymes, osteogenesis, and osteoporosis" as high as 404 items resulted. Dental caries was associated with an order of magnitude fewer studies than the chronic bone disease, osteoporosis. This observation motivated this review. MATERIAL AND METHODS A comprehensi...