The complete amino acid sequence of Neurospora crassa cytochrome c has been determined. The molecule consists of a single polypeptide chain containing 107 residues. The NH2-terminal residue is glycine, and the COOH-terminal residue is alanine. There are 39 amino acid differences between the cytochromes of N. crassa and those of the yeast Saccharomyces cerevisiae, whereas there are 43 amino acid...

In Neurospora crassa, sequence-specific inhibition of endogenous genes can be induced by the introduction of transgenic DNA homologous to the target gene, through the mechanism of post-transcriptional gene silencing (PTGS) known as quelling. The application of this strategy to inactivate genes in N. crassa has, to date, been restricted by a limited silencing efficiency and instability of the si...

This paper analyses relationships between relative growth rate (), seed mass, biomass allocation, photosynthetic rate and other plant traits as well as habitat factors (rainfall and altitude) in 20 wild species of Aegilops L. and one closely related species of Amblyopyrum (Jaub. & Spach) Eig., which differ in ploidy level (diploid, tetraploid and hexaploid). The plants were grown hydroponica...

Background and Aims Bread wheat (Triticum aestivum) has been through a severe genetic bottleneck as a result of its evolution and domestication. It is therefore essential that new sources of genetic variation are generated and utilized. This study aimed to generate genome-wide introgressed segments from Aegilops speltoides. Introgressions generated from this research will be made available for ...

Crosses between different genera of plants are rare and a satisfactory cytological study of such hybrids has never been made. A comparison of chromosome behavior in genus and species crosses is of interest in connection with a comparison of genetic and taxonomic relationships. A cytological analysis should also show the reason why crosses of genera are almost completely or quite sterile and whe...

Relief of an auxotrophic requirement for uridine in Aspergillus nidulans strain G191 has been achieved by transformation with a segment of Neurospora crassa DNA containing the corresponding gene coding for orotidine-5'-phosphate decarboxylase. The mitotic stability of such transformants suggests that the DNA has integrated into the genome. Southern hybridisation analysis of DNA isolated from tr...

genetic drift of germplasm in cultivated wheat is good motivation for studying genetic diversity in its wild relatives. in this study, r-rap, a new retrotransposon-based marker, was applied to assess the structure and genetic diversity of 30 accessions of aegilops sp. originating from different geographical regions of iran. the 15 r-rap primer combinations revealed a total of 338 bands ranging ...

Ornithine decarboxylase (ODC) of the fungus Neurospora crassa, encoded by the spe-1 gene, catalyzes an initial and rate-limiting step in polyamine biosynthesis and is highly regulated by polyamines. In N. crassa, polyamines repress the synthesis and increase the degradation of ODC protein. Changes in the rate of ODC synthesis correlate with similar changes in the abundance of spe-1 mRNA. We ide...

Stress genes are differentially expressed during the development of Neurospora crassa and other fungi. Large amounts of constitutive heat shock protein 70 (HSC70) are found in dormant conidia of N. crassa, whereas little mRNA of the related glucose-regulated protein (grp78) is detected. It is, however, not generally clear whether heat shock protein or mRNA is preferentially stored in dormant sp...