Objectives: After revolutionized treatment of erectile dysfunction (ED) with phosphodiesterase-5 inhibitors (PDE5) inhibitors, approximately 30% of patients are non-responsive. An important cause of this is vascular and smooth muscle dysfunction, as well as nerve atrophy. Stem cells are characterized by anti-inflammatory activities, as well as possibility of differentiating into tissue relevant...

OBJECTIVES Although an alveolar air leak (AAL) after pulmonary resection is a troublesome complication that diminishes a patient's quality of life and increases medical costs, current treatment and preventive methods for AAL are not effective. Therefore, we transplanted adipose-derived stem cells (ASCs) to the damaged visceral pleura to facilitate the regeneration of mesothelial cells and inves...

Diabetic retinopathy (DR) is the leading cause of blindness in working-age adults. Early stage DR involves inflammation, vascular leakage, apoptosis of vascular cells and neurodegeneration. In this study, we hypothesized that cells derived from the stromal fraction of adipose tissue (ASC) could therapeutically rescue early stage DR features. Streptozotocin (STZ) induced diabetic athymic nude ra...

introduction: mesenchymal stem cells (mscs) hold great promise in the field of regenerative medicine.although originally isolated from bone marrow, mscs have since been obtained from a variety of adult and neonatal tissues including the adipose tissue. stemness and multipotential features of mesenchymal stem cells (msc) has been highlighted in many studies but there are many dark aspects in exc...

Abstract Background Crohn’s disease (CD) is characterized by persistent inflammation and ulcerations at the small or large bowel, provoking chronic low-grade systemic inflammation. Adipose tissue (AT) believed to play an active role in pathogenesis of CD, as expansion mesenteric fat attached inflamed segments intestine, also known “creeping fat,” a hallmark that seems be directly related activi...

Stem cells derived from the subcutaneous adipose tissue of debrided burned skin represent an appealing source of adipose-derived stem cells (ASCs) for regenerative medicine. Traditional tissue culture uses fetal bovine serum (FBS), which complicates utilization of ASCs in human medicine. Human platelet lysate (hPL) is one potential xeno-free, alternative supplement for use in ASC culture. In th...

Integration of stem cells to injured tissues requires an appropriate delivery device and scaffolding system. In the present study we have developed an in vitro strategy to load and release adipose-derived mesenchymal stem cells (ASC) from chitosan microspheres (CSM) into a collagen gel scaffold. Porous CSM of uniform size and composition were prepared and used as a stem cell carrier. ASC were a...

BACKGROUND Adipose-derived stem cells (ASCs) are mesenchymal stem cells that have recently been applied to tissue repair and regeneration. Keratinocytes and dermal fibroblasts play key roles in cutaneous wound healing. OBJECTIVE We investigated the paracrine effects of ASCs on HaCaT cells (i.e., immortalized human keratinocytes) and human dermal fibroblasts to explore the mechanism of the eff...