dynamic adhesions within the cell. The data from Gupton and Waterman-Storer (2006) suggest that low fibronectin is suboptimal for migration because there are too few adhesions even though the adhesions are dynamic. High fibronectin is suboptimal because the adhesions are too stable and do not signal. Cells on intermediate fibronectin have a high number of small, dynamic adhesions of the kind th...

BACKGROUND Postoperative intra-abdominal adhesions are common complications after abdominal surgery. The exact molecular mechanisms that are responsible for these complications remain unclear, and there are no effective methods for preventing adhesion formation or reformation. The aim of the study reported here was to investigate the preventive effects and underlying potential molecular mechani...

INTRODUCTION Dense lower abdominal adhesions are known to cause urinary frequency by restricting expansion of the bladder. However, since preoperative diagnosis of adhesions has been difficult and there are multiple other causes of urinary frequency, such patients may go undiagnosed. With the improving resolution of ultrasound, ever smaller pathologic structures may be visualized, particularly ...

Fibroblast migration involves complex mechanical interactions with the underlying substrate. Although tight substrate contact at focal adhesions has been studied for decades, the role of focal adhesions in force transduction remains unclear. To address this question, we have mapped traction stress generated by fibroblasts expressing green fluorescent protein (GFP)-zyxin. Surprisingly, the overa...

Adhesion and detachment are coordinated critical steps during cell migration. Conceptually, efficient migration requires both effective stabilization of membrane protrusions at the leading edge via nascent adhesions and their successful persistence during retraction of the trailing side via disruption of focal adhesions. As nascent adhesions are much smaller in size than focal adhesions, they a...

In this study, we examined the effect of diaphragmatic peritoneal lymphatic function on the formation of peritoneal adhesions. A two phased design was used in fifty-two Wistar albino female rats. In the first phase (n = 12), the effects of diaphragmatic peritoneum damage model on the cecum and the terminal ileum were evaluated. In the second phase, the rats (n = 40) were divided into two equal ...

The six serine/threonine kinases in the p21-activated kinase (PAK) family are important regulators of cell adhesion, motility and survival. PAK6, which is overexpressed in prostate cancer, was recently reported to localize to cell-cell adhesions and to drive epithelial cell colony escape. Here we report that PAK6 targeting to cell-cell adhesions occurs through its N-terminus, requiring both its...

We have investigated whether an oxygen/ozone (95%O2/5%O3) mixture would have potential against the formation of experimental postsurgical peritoneal adhesions. In two groups of rats, one control intraperitoneally injected with 3 mL/rat of O2 and one intraperitoneally injected with oxygen/ozone mixture (3 mL/rat equivalent to 300 μg/kg ozone), we induced a midline laparotomy and an enterotomy at...

BACKGROUND In modern obstetrics caesarean sections are commonly used and in all caesarean sections either peritoneal closure is done or not. This cases series was conducted to record outcomes in our setup. METHODS Cases of caesarean sections were followed for adhesions and non-adhesions in peritoneal closure and non-closure cases from 1't January to December 2011 at DHQ Hospital Haripur. In n...

Integrin-dependent adhesions are mechanosensitive structures in which talin mediates a linkage to actin filaments either directly or indirectly by recruiting vinculin. Here, we report the development and validation of a talin tension sensor. We find that talin in focal adhesions is under tension, which is higher in peripheral than central adhesions. Tension on talin is increased by vinculin and...