What is adenovirus? Adenoviruses were first isolated in human adenoids, from which the name is derived. Adenoviruses are medium-sized (90–100 nm), nonenveloped (naked) icosahedral viruses composed of a nucleocapsid and linear, non-segmented double stranded (ds) DNA genome which is about 36kb long. The genes of Adenovirus are divided into two phases, early and late phases. Genes of early phase a...

This JAMA Patient Page explains how the Johnson & COVID-19 vaccine, likely to be third SARS-CoV-2 vaccine approved for widespread use, uses an adenovirus 26 vector deliver coronavirus DNA host cells produce spike proteins and safely effectively induce immunity.

The author describes the case of a 3-year-old boy presenting with cough and fever that had been persisting for seven days not respondent to amoxicillin. Neutrophilic leukocytosis diarrhoea were also present. diagnosis adenovirus infection was finally made, confirmed by serological test.

Systemic application of first-generation adenovirus induces pathogenic effects in the liver. To begin unraveling the mechanisms underlying early liver toxicity after adenovirus infusion, particularly the role of macrophage activation and expression of viral genes in transduced target cells, first-generation adenovirus or adenovirus vectors that lacked most early and late gene expression were ad...

PURPOSE Adenovirus-mediated gene therapy combined with radiation is expected to be a new approach to treat pancreatic cancer. However, there are no reports of definitive effects of radiation on adenovirus-mediated gene therapies. In the present study, we investigated the effect of radiation on the transduction efficiency of an adenovirus-based gene therapy. EXPERIMENTAL DESIGN We used adenovi...

Adenoviral gene transfer is a valuable tool in molecular biology research. In order to be an efficient and safe vector, adenovirus structure and infection mechanism as well as molecular biology of the used transgene need to be well studied. The aim of this study was to evaluate the role of adenovirus as a gene transfer vector from several perspectives. Adenovirus uses receptor-mediated endocyto...

We recently demonstrated how various enveloped viruses can be efficiently concentrated using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). However, the exact mechanism of interaction between the virus particles and anionic beads remains unclear. To further investigate whether these magnetic anionic beads specifically bind to the viral envelope, we exa...

AIMS To investigate adenovirus pulmonary infections in bone marrow transplant (BMT) recipients. METHODS Formalin fixed, paraffin wax embedded lung tissue was examined from 13 necropsy cases after BMT using PCR and in situ hybridisation to detect adenovirus DNA. The E1A region of the adenoviral genome was targeted for PCR. In situ hybridisation was performed only in the PCR positive cases. R...

As an approach to the purification of adenovirus-encoded DNA replication proteins, we have developed in vitro complementation assays that make use of viral mutants defective in DNA replication in vivo. Nuclear extracts prepared from cells infected with H5ts36 or H5ts125, two such mutants belonging to different complementation groups, were found to be defective in viral DNA replication in vitro....