A phospholipase A1 bound tightly to the membranes of Mycobacterium phlei cells was purified approximately 500fold to near homogeneity by extraction with Triton X-100, delipidation with organic solvents, solubilization with sodium dodecyl sulfate, column chromatographies on Sephadex G-200 in the presence of sodium dodecyl sulfate and on DEAE-cellulose in the presence of BRIJ 58, and sodium dodec...

Ion chromatography (IC) developed by Small et al . [1] is widely recognized as a simple, powerful and convenient analytical technique for the determination of various low−molecular−weight ionic compounds [2, 3]. Firstly, IC with conductimetric detection (IC−CD) was mainly applied for the determination of inorganic anions. This is because a lightly sulfonated styrene−divinylbenzene co−polymer (S...

Growth-associated protein-43 (GAP-43) is believed to be palmitoylated near the N terminus and the modification is assumed to be involved in the membrane anchoring of the protein. However, GAP-43 isolated from bovine brain is not palmitoylated as shown by mass spectrometric analysis, but still retains the ability to bind phospholipids, suggesting that other parts of the molecule are involved in ...

Poly- and perfluoroalkyl substances (PFASs) have been detected in an increasing number of water supplies. In many instances, the contamination is associated with the use of PFAS-containing aqueous film-forming foams (AFFF) in firefighting activities. To investigate the potential for remediating AFFF contamination in groundwater with heat-activated persulfate, PFAS oxidation and the generation o...

AIM The objective of this study is to gain a better understanding of the antimicrobial properties of the mucus extract of snakehead fish, Channa striatus against selected human and fish pathogenic microbes. MATERIALS AND METHODS The fish mucus samples were extracted with crude, acidic and aqueous solvents to identify potential antimicrobial agents including aqueous and acid soluble compounds....


 Prunus Arminica.L seeds of Hunza cultivar were defatted with chloroform, methanol mixture (2:1v/v) by ultrasonic assisted extraction technique. The lipase and phospholipase extracted, incubated at 400C pH 6 in citrate buffer for 1 hour. lipolytic activities enzymes measured different temperatures i,e 30-700C , 3-9 solvents n-heptane Di-isopropyl ether Cyclo hexane. 10 % emulsion lecithin...

This study aimed to evaluate the properties of soda lignin obtained from an unconventional and abundant waste - rambutan peel since has been shown as most promising natural organic feedstock alternative petroleum for polymer science. FTIR analysis pH measurements have confirmed acidic form lignin, which solubility in a wide range polarities so many solvents, making its insertion easy on polymer...

peroxyoxalate system includes an oxalate ester, peroxide and a fluorescer, the energy resulted from the reaction of oxalate ester and peroxide is transferred to fluorescer and cause it to fluorsc. the effect of reactants chemical structure, their concentration, solvent media (polarity and viscosity) and the temperature, on the intensity of the illuminated light with respect to time have been st...

Adhesion underwater is a major challenge. Mussel-inspired complex coacervates functionalized with L-3,4-dihydroxyphenylalanine (L-DOPA) are proposed for adhesives through versatile chemistry of DOPA, however, simple, efficient, controllable, and nontoxic procedures to harness them still under investigation. In this study, inspired from the mussel byssus formation process, coacervate formed by s...