OBJECTIVE
To examine whether in vitro exposure of mouse middle ear epithelial cells (mMEECs) to conditions that mimic physiologic reflux upregulates Muc5b gene expression and alters cell viability.
DESIGN
In vitro mMEEC model.
SETTING
Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC.
PARTICIPANTS
Cells from the immortalized mMEEC line.
MAIN OUTCOM...