The purpose of the work presented here was to evaluate the influence of solution composition and analyte characteristics on responsiveness to analysis with negative ion electrospray ionization mass spectrometry. The responses of a series of structurally diverse acidic molecules were compared in various solvents. Response was generally observed to be higher in methanol than acetonitrile and resp...

The keratins expressed in cultured rat cervical epithelial cells were analyzed by using two-dimensional gel electrophoresis and monoclonal antikeratin antibodies and were compared with those expressed in the rat cervix in vivo in the presence and absence of estrogen stimulation. The cervical epithelium in vivo responds to estrogen stimulation with alterations in keratin composition. In response...

Increasing antibiotic resistances of numerous pathogens mean that myxobacteria, well known producers of new antibiotics, are becoming more and more interesting. More than 100 secondary metabolites, most of them with bioactivity, were described from the order Myxococcales. Especially new myxobacterial genera and species turned out to be reliable sources for novel antibiotics and can be isolated ...

At least 14 ascorbate oxidase (AO) isoenzymes with isoelectric points in the range of pH 3 /10 have been identified and their expression profile studied in various Cucurbitaceae tissues and during development and ripening of melon fruit (Cucumis melo L. var. reticulatus). At least four acidic AO isoenzymes were mainly located in the apoplastic fraction, whereas 10 basic AO isoenzymes were found...

Red spruce (Picea rubens Sarg.) seedlings were exposed repeatedly at a field site or in a greenhouse~to acidic mist containing the major sulfur and nitrogen pollutants of wet deposition in the eastern U.S.: sulfate alone, nitrate alone or with ammonium ion, and a combination of these ions. Acidities and ion concentrations ranged from below the mean to above the maximum concentration for cloudwa...

a variety of alcohols readily add to 3,4-dihydro-2h-pyran under solvent free conditions in the presence of catalytic amount of acidic ionic liquid [et3n(ch2)4so3h][ots] to afford the corresponding tetrahydropyranyl ethers in good to excellent yields at room temperature. the use of this procedure allows easy separation of the desired products from ionic liquid and recycling the ionic liquid. som...

background: induced pluripotent stem cells (ipscs) can be obtained from autologous cells for therapeutic purposes. so, far, many studies have been done to produce induced pluripotent cells by transferring specific pluripotency genes using different methods. in this study, pluripotency gene expression induced by external stresses was assessed in cord blood mononuclear cells. methods: in this exp...

Bismuth and antimony are semimetals with a rhombohedral crystal structure and similar lattice parameters. Bi12xSbx alloys exhibit unusual electronic properties and are of interest for thermoelectric and magnetoelectronic devices. In this paper, we show that Bi12xSbx alloys can be electrodeposited from acidic chloride-based solutions and that a homogenous solid solution is obtained across the en...

INTRODUCTION The aim of this study was to investigate the changes in the surface structure and chemical composition after applying bleaching agents to completely hardened mineral trioxide aggregate. METHODS A total of 12 samples of MTA blocks were divided into three groups, two different bleaching agents, and a control group. The surface structure was observed using a scanning electron micros...

6.1 Overview In Chapter 5 a new universal cloning strategy for the production of specifically designed surface-active PAA’s was introduced. Here, we apply this strategy to the production of a unique surface-active PAA designed to form brush layers on aluminum oxide surfaces. This chapter discusses the procedure taken in assembling the gene encoding for a PAA diblock consisting of a long zwitter...