Introduction: Mycobacterium tuberculosis is the second most infectious cause of death in adults globally and major health trouble Nepal. The diagnosis pulmonary mainly carried by acid-fast bacilli smear microscopy, culture on Lowenstein–Jensen media, GeneXpert MTB/RIF assay. This study aimed to evaluate Acid-Fast Bacilli stain used for tuberculosis.
 Methods: A cross-sectional was performe...

According to WHO one third of the world population have tuberculosis. The present study was undertaken to compare the efficacy of flurochrome(FI) stain with conventional Z-N stain in the diagnosis of pulmonary & extrapulmonary tuberculosis. 388 cases of suspected pulmonary tuberculosis were included in the study. All samples were screen for Acid Fast Bacilli (AFB) by Z-N & Fluorescent staining ...

Tuberculous pleuritis (TP) is one of the most common extra-pulmonary tuberculosis form. Because tuberculous pleurisy hard to diagnose due slow course disease and lack specificity in symptoms diagnostic methods. In that reason, we need multidisciplinary approach efficient biomarkers. Acid-fast bacilli (AFB) staining, cultures pathophysiological biopsy finding from majority patients are positive ...

The aim of this study was to compare Fluorescent(auraminO rhodamine,AO)method ,Ziehl-Neelsen (ZN hot method)and kinyoun(cold method) staining techniques for the diagnosis of Mycobacterium tuberculosis. Materials And Methods: 186(on 3 consecutive days) sputum samples were collected from 62 suspected pulmonary tuberculosis patients, processed by petroffs method, and subjected to direct microscopy...

Back ground: Acid fast bacilli (AFB) positivity using modified Ziehl Neelsen (MZN) staining and jar method was studied in sputum samples obtained by spot morning (SM) and same day (SS2) approaches. Methods: Three specimens (spot, second spot and morning) were collected in 252 patients, duplicate smears were prepared and stained by MZN staining and jar method. Results: Smear positivity was 34 (1...