Previous reports from this laboratory (R. K. Rasmussen and H. P. Klein, Biochem. Biophys. Res. Commun. 28:415, 1967) indicated that many organic compounds can stimulate the acetylcoenzymeA (CoA) carboxylase of Saccharomyces cerevisiae strain LK2G12. The enzyme from this organism appears to differ from that reported by Matsuhashi et al. (Biochem. Z. 340:243, 1964; Biochem. Z. 340:263, 1964), who...

Acetyl-coA carboxylase isolated from freezeclamped livers of fed rats has relatively low phosphate content (6.0 mol of PJmol of subunit) and high specific activity (3.6 unitslmg in the absence of citrate). The enzyme from rats fasted for 12, 18, 24, and 48 h exhibited decreasing specific activities of 2.75, 1.86, 1.7, and 0.9 unitslmg, respectively. Citrate activated all preparations of carb...

2',3'-O-(2,4,6-Trinitrophenyl) adenosine 5'-triphosphate (TNP-ATP) is a fluorescent analogue of ATP. MgTNP-ATP was found to be an allosteric activator of pyruvate carboxylase that exhibits competition with acetyl CoA in activating the enzyme. There is no evidence that MgTNP-ATP binds to the MgATP substrate binding site of the enzyme. At concentrations above saturating, MgATP activates bicarbona...

abstract in order to determine efficacy of some registered graminicides in control of resistant and susceptible ryegrass biotypes, two greenhouse trials were conducted at weed research department of iranian plant protection research institute during 2007. experimental design was completely randomized design with eight replications. the susceptible and resistant biotypes were treated by 19 herbi...

Andrimid is a hybrid nonribosomal peptide-polyketide antibiotic that blocks the carboxyl-transfer reaction of bacterial acetyl-CoA carboxylase (ACC) and thereby inhibits fatty acid biosynthesis with submicromolar potency. The andrimid biosynthetic gene cluster from Pantoea agglomerans encodes an admT gene with homology to the acetyl-CoA carboxyltransferase (CT) beta-subunit gene accD. Escherich...

Ignicoccus hospitalis is an autotrophic hyperthermophilic archaeon that serves as a host for another parasitic/symbiotic archaeon, Nanoarchaeum equitans. In this study, the biosynthetic pathways of I. hospitalis were investigated by in vitro enzymatic analyses, in vivo (13)C-labeling experiments, and genomic analyses. Our results suggest the operation of a so far unknown pathway of autotrophic ...

An investigation was made of the interaction of pyruvate carboxylase with its allosteric effector, acetyl-CoA, and the velocity profile of the deacylation of acetyl-CoA as a function of acetyl-CoA concentration indicated that this ligand does not bind to this enzyme in a positive homotropic co-operative manner. An examination was therefore made of the factors that contribute to the sigmoidicity...

Holocarboxylase synthetase (HCS) catalyzes the covalent attachment of biotin to five biotin-dependent carboxylases in human cells. Multiple carboxylase deficiency (MCD) is a life-threatening disease characterized by the lack of carboxylase activities because of deficiency of HCS activity. Here, we report the obligatory participation of HCS in the biotin-dependent stimulation of the level of HCS...

of medium chain fatty acids (MCFAs; C 8 –C 12) requires their activation inside peroxisomes by an acyl-CoA syn-SFB Biomembrane Research Center Institut fü r Biochemie und Lebensmittelchemie thetase, Faa2p. Myristoylation of membrane-associated proteins is catalyzed by Nmt1p (for review on lipid syn-Technische Universitä t Graz Petersgasse 12 thesis in yeast, see Kohlwein et al., 1996). S. cerev...