Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

Biological sensor platforms usually combine a recognition element (e.g. antibody, recombinant protein/peptide, or oligonucleotide/oligonucleoside) that binds a target molecule (e.g. protein, DNA fragment, or whole cell) with a transduction/ output scheme that provides a readable signal. A class of proteins called protein switches represent a unique platform for biosensors since they combine bot...

in this paper, the writers have collected max weber's views on the worldly approach of islam from his book sociology of religion and presented as a coherent discussion. the writers have then discussed the arguments weber has put forward to prove his point and criticized weber's theory and his personality as a critic. as far as his theory is concerned, the writers have tried to provide answers t...

advisable to devote a special article to this subject. This will prove a great boon to, seekers after truth. The surgical summaries include one on the use of refrigerated blood for blood transfusions; one on intractable sciatica due to retropulsed nucleus pulposus; one on sympathectomy and its uses; and many others of general surgical interest. Two sections are of interest to everyone. One on t...

jami’s baharestan is one of the most valuable persian literary works; it embodies a lot of fruitful stories and discourses, whose most sources are arabic. though one may find out from jami’s words that he has not used the literary works of his predecessors, an investigation on arabic sources may lead one to lots of background works of the stories of this book. in this paper, we have identified ...

University of Alberta set out to build a world checkers champion. He succeeded. In 1994, CHINOOK, the program written by Schaeffer and his group, won the Man versus Machine World Checkers Championship. (Although CHINOOK has now mostly retired from championship checkers, it is still possible to play CHINOOK. To play CHINOOK or just see information about CHINOOK and checkers in general, go to www...

James Yorke won the 2003 Japan Prize for his work in the field of chaos theory. This book was compiled by four of his best-known collaborators in honor of his 60th birthday and contains papers by various authors on chaos and chaotic attractors. The papers are organized around the topics of “natural” invariant measure and fractal dimension of the associated chaotic attractors. A few of the paper...

this book makes abundantly clear the sustained freshness of snyder path-breaking and dauntingly original work, which has long set the agenda for the systematic explanation of foreign policy decisions. thevery thoughtful essays prepared for the present volume demonstrate dramatically the continued centrality of the issues snyder and his associates raised more than 40 years ago. prescient in its ...