In this work we have identified, using mass spectrometry, two laccases produced by Leucoagaricus gongylophorus. One of them, Lac1Lg, was isolated, purified and characterized. Lac1Lg, a monomeric enzyme, was studied using ABTS and syringaldazine substrates. Lac1Lg presented kcat/Km almost threefold higher for syringaldazine than for ABTS, showing a higher catalytic efficiency of Lac1Lg for syrin...

Considering the crucial role played by microRNAs (miRNAs) in biological processes, we developed a novel strategy for simple and colorimetric detection of miRNA by combining target amplification with DNAzyme. Throughout the work, a 22-nt oligonucleotide sequence was used as a model analyte. A label-free hairpin probe (HP) was used as a simple platform for sensing the target. In the presence of t...

Felicia muricata is a medicinal plant used for the management of different human and livestock diseases in the Eastern Cape Province of South Africa. The antioxidant potential of the leaves from this herb was investigated using its water, methanol, acetone and ethanol extracts. All the extracts were rich in phenols, proanthocyanidins and flavonols but low in flavonoids. The water extract exhibi...

To investigate the biological activities of anthocyanins, which are induced by cadmium in A. imbricata, the antioxidant properties of anthocyanins were investigated using various antioxidant assays, namely 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azinobis-(3-ethylbenzthiazoline-6sulfonic acid) (ABTS) radical scavenging activity, reducing power, and β-carotene bleac...

This study reports decolorization of Rhodamine B and Congo red by a partially purified laccase from Lentinus polychrous Lév. in the presence or absence of 2,2-azinobis (3-ethylbenzothiazoline-6sulfonate) diammonium salt (ABTS) as a redox mediator. The decolorization study showed that about 90% of Rhodamine B could be decolorized within 52 h, whereas Congo red could be decolorized more than 75% ...

During the course of horseradish peroxidase-mediated oxidation of either o-dianisidine or 2-2'-azino-di(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS), no O2 consumption took place. When isonicotinic acid hydrazide (isoniazid) (INH) was included in the reaction mixture, O2 was consumed in amounts linearly related to the INH concentration. Nicotinic acid hydrazide at equimolar concentrations ind...

The deposits of Bor and Cukaru Peki are important contributors to the Apuseni–Banat–Timok–Srednogorie (ABTS) belt’s metallogenic endowment. We use decision tree random forest algorithms applied zircon geochemistry data from Bor, a selection other localities within ABTS. resulting predictions, supported by high scores on test set predictions for algorithm, suggest that it is possible fingerprint...