OBJECTIVE The direct antiglobulin test (DAT) is an important tool for identification of haemolytic disease of the newborn (HDN) caused by erythrocyte immunization. Although this test has been used for decades, accurate insights into its diagnostic properties and optimal use in the diagnosis of HDN are limited. We aimed to gain more insight into the diagnostic properties of the DAT for HDN by co...

In a concurrent study of the serum activity of glutamate-oxalacetate transaminase (GO-T), glutamate-pyruvate transaminase (GP-T), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) in normal newborn infants ranges of normal values for these enzymes have been defined (King and Morris, 1961). In the present report the activity of these enzymes in relation to neonatal jaundice and in prema...

Delayed donor red cell engraftment and pure red cell aplasia (PRCA) are well-recognized complications of major ABO-incompatible hematopoietic stem cell transplantation (SCT) performed by means of myeloablative conditioning. To evaluate these events following reduced-intensity nonmyeloablative SCT (NST), consecutive series of patients with major ABO incompatibility undergoing either NST (fludara...

Donor-recipient ABO and/or HLA incompatibility used to lead to donor decline. Development of alternative transplantation programs enabled transplantation of incompatible couples. How did that influence couple characteristics? Between 2000 and 2014, 1232 living donor transplantations have been performed. In conventional and ABO-incompatible transplantation the willing donor becomes an actual don...

BACKGROUND ABO incompatible kidney transplantation (ABOi-KT) is an important approach for overcoming donor shortages. We evaluated the effect of ABOi-KT on living donor KT. METHODS Two nationwide transplantation databases were used. We evaluated the impact of ABOi-KT on overall living donor transplant activity and spousal donation as subgroup analysis. In addition, we compared the clinical ou...

The minimum number of IgG anti-A (or anti-B) molecules detectable on A or B red cells by the antiglobulin reaction was found to be the same-that is, about 150 molecules per red cell-with newborn as with adult cells. Furthermore, the ratio of anti-IgG bound to IgG anti-A (or anti-B) molecules was the same whether the anti-A (or anti-B) molecules were present on newborn or on adult cells and was ...

Determinations were carried out of the ABO blood groups and Rhesus phenotypes of a series of 142 children with Perthes' disease. Where possible their parents and sibs were also grouped. Sera from the mothers of affected children were examined for the presence of Rhesus antibodies. No apparent association was demonstrated between Perthes' disease and any ABO phenotype. The disease is also appare...