UNLABELLED The present work was AIMED on the study of human beta-defensin 3 (hBD-3) gene expression in A431 cell line and human vulval tumors. MATERIALS AND METHODS Twenty surgical specimens of both malignant and conventionally normal human vulval tissues have been analyzed for the presence of hBD-3 mRNA by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). In vitro e...

Aquaporin 3 (AQP3) and phospholipase D2 (PLD2) are abnormally expressed and/or localized in squamous cell carcinoma (SCC). AQP3 transports glycerol to PLD2 for the synthesis of lipid second messenger, which can mediate the effect of the AQP3/PLD2 signaling module in the regulation of keratinocyte proliferation and differentiation. However, the role of the AQP3/PLD2 signaling module in the patho...

UNLABELLED Significant liver uptake often limits the clinical application of radiolabeled antibodies in radioimmunodetection. The purpose of this study was to evaluate the gamma-imaging properties of an antiepidermal growth factor receptor (EGFR) antibody, C225, conjugated with heterofunctional poly(ethylene glycol) (PEG) with 1 terminus of the polymer attached to a radiometal chelator, diethyl...

Calotropis gigantea (Linn) and Amoora rohituka (Roxb.) have long been used in folkloric medicine for the treatment of various ailments in Bangladesh. The present study was designed to investigate the cytotoxicity of isolated compounds from these two plants. Two compounds, named β-boswellic acid (compound-1) and 2-methoxy-14-calamenenone (compound-2) were isolated from the flowers of Calotropis ...

Epidermal growth factor (EGF) receptor protein has been purified in a single high-yield step by immunoaffinity chromatography of extracts of A431 cells. A monoclonal antibody directed against the EGF binding site of the receptor was immobilized to Sepharose 4B as a specific immune absorbent and competitive elution with EGF was used to obtain purified EGF receptor protein with tyrosine kinase ac...

Tyrosine-specific protein kinase activity of the epidermal growth factor (EGF) receptor, pp60v-src and pp110gag-fes was inhibited in vitro by an isoflavone genistein. The inhibition was competitive with respect to ATP and noncompetitive to a phosphate acceptor, histone H2B. By contrast, genistein scarcely inhibited the enzyme activities of serine- and threonine-specific protein kinases such as ...

We report application of two-photon excitation of europium chelates to immunolabeling of epidermal growth factor receptor (EGFR) cell surface proteins on A431 cancer cells. The europium chelates are excited with two photons of infrared light and emit in the visible. Europium chelates are conjugated to antibodies for EGFR. A431 (human epidermoid carcinoma) cells are labeled with this conjugate a...

A new diaminocarbene cis-palladium(II) complex containing a 2-aminobenzoxazole ligand was synthesized by reacting cis-[PdCl2(CNCy)2] and 2-aminobenzoxazole. The structure composition of the obtained were proven NMR spectroscopy high-resolution mass spectrometry. cytotoxicities structurally similar palladium(II) complexes 2-aminothiazole tested against human cancer cells various histogenesis (MC...