Factor VIII (fVIII) circulates as a heavy chain/light chain (A1-A2-B/ap-A3-C1-C2) heterodimer. The 41-residue light chain activation peptide, ap, is cleaved from fVIII during proteolytic activation by thrombin or factor Xa. We constructed 7 active recombinant hybrid B-domainless human/porcine fVIII molecules that contained combinations of porcine sequence replacements within the A2, ap-A3, and ...

A bacterial strain Pseudomonas sp. a3 capable of degrading nitrobenzene, phenol, aniline, and other aromatics was isolated and characterized. When nitrobenzene was degraded, the release of NH(4) (+) was detected, but not of NO(2) (-). This result implied that nitrobenzene might have a partial reductive metabolic pathway in strain a3. However, aniline appeared as one of the metabolites during th...

The focus of this paper is to present students’ perceptions of the recently developed A3 method for problem solving in the healthcare environment. The students were all employees of a large healthcare provider and were enrolled in a customized healthcare EMBA Program. Each student in the class was required to complete an individual A3 Project in order to improve a process at the department for ...

Recent Mössbauer and EPR studies of two ferredoxins and of aconitase have given evidence for a three-iron cluster, probably of a [3Fe-3S] type. The studies of the oxidized EPR-active centers have shown that the three iron sites are characterized by significantly different magnetic hyperfine coupling constants. For the ferredoxin from Azotobacter vinelandii, for instance, we have observed A1 = -...

BACKGROUND In the ASE/AIE-mediated genetic mechanism for age-related gene regulation, a recently identified age-related homeostasis mechanism, two genetic elements, ASE (age-related stability element) and AIE (age-related increase element as a stem-loop forming RNA), play critical roles in producing specific age-related expression patterns of genes. PRINCIPAL FINDING We successfully identifie...

Adoptive immunotherapy using TCR-engineered PBLs against melanocyte differentiation Ags mediates objective tumor regression but is associated with on-target toxicity. To avoid toxicity to normal tissues, we targeted cancer testis Ag (CTA) MAGE-A3, which is widely expressed in a range of epithelial malignancies but is not expressed in most normal tissues. To generate high-avidity TCRs against MA...

HE genetic structure of Streptomyces coelicolor A3 (2), the only strain among the actinomycetes for which a genetic map has been constructed, was studied in detail by HOPWOOD (1967) and SERMONTI (1 969). For genetic analysis, HOPWOOD and SERMONTI utilized the capacity of mutant strains of A3 (2) to undergo genetic recombination. To enlarge the possibilities of genetic analysis of the actinomyce...

Judiciously classifying phase-A subtypes in cyclic alternating pattern (CAP) is critical for investigating sleep dynamics. Phase-amplitude coupling (PAC), one of the representative forms of neural rhythmic interaction, is defined as the amplitude of high-frequency activities modulated by the phase of low-frequency oscillations. To examine PACs under more or less synchronized conditions, we prop...

Anthraquinones are of significant interest due to their biological activity, coloring properties, and synthetic applications. Here, we describe a mild convenient method for modification 1-ethynyl-4-hydroxyanthraquinone that was obtained from the Sonogashira cross-coupling reaction 1-hydroxy-4-iodoanthraquinone with alkynes. The copper(I) catalyzed one-pot three-component (A3-coupling) new secon...