background : the protozoan parasites cryptosporidium spp. and giardia are known to occur widely in both raw and drinking waters. they are two of the causative agents of waterborne out-breaks of gastroenteritis throughout the world. in the present study, a pcr assay and fa were developed for detection of cryptosporidium oocysts and giardia cyst in environmental samples. methods : we have detecte...

References: 1) Chung.D.W., Harris,J.E. and Davie.E.W. (1991) In Liu,C.Y. and Chien,S. (eds) Fibrinogen, Thrombosis, Coagulation and Fibrinolysis. Plenum Press, NY. pp. 39—48. 2) MUls,K.A., Buetow,K.H., Xu,Y., Weber.J.L., AJtherr,M.R., Wasmuth,J.J. and Murray,J.C. (1992) Genomics, 14, 209-219. Source/Description: The (AC)n dinucleotide repeat sequence was isolated from a human fetal retinal cDNA...

viral symptoms indicative of iris yellow spot virus (iysv) were observed on onion in several fields near chenaran in khorasan razavi province. mechanical inoculation of herbaceous hosts with onion sap extracts from symptomatic plants showed similar symptoms to those described for iysv. the mechanically transmitted virus reacted only with antisera specific to iysv in das-elisa but not with antis...

infectious bursal disease (ibd) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. vp2 is the major host-protective protein of infectious bursal disease virus (ibdv). the encoding region of vp2 protein was pcr amplified from a plasmid containing a cdna fragment of large genomic segment of ibdv, strain d78. thi...

In a 16S rRNA gene-directed multiplex PCR, Prevotella intermedia- and Bacteroides forsythus-specific reverse primers were combined with a single conserved forward primer. A 660-bp fragment and an 840-bp fragment that were specific for both species could be amplified simultaneously. A total of 152 clinical samples, subgingival plaque and swabs of three different oral mucosae, from 38 periodontit...

A methodological approach is proposed to select rapidly DNA sequences characterized by a well defined specificity and potentially interesting to be used as diagnostic probes or as taxonomic and phylogenetic markers. A fragment amplified from a diversified sample of Trypanosoma cruzi stocks by the RAPD (random amplified polymorphic DNA) method with the A8 primer, previously found to be monomorph...

Background Environmental and cellular inappropriate conditions can cause damages to cells nucleotide poll. Deamination and oxidation damages interfere with cell�s vital reactions. Inosine triphosphate pyrophosphatase (ITPA), an evolutionary conserved enzyme, plays a critical role in elimination of non-canonical bases. In human genome, the ITPA gene is located on chromosome 20 short arm and tran...

A polymerase chain reaction (PCR)-based test was developed for the detection of Salmonella typhi in the blood specimens from patients with typhoid fever. Two pairs of oligonucleotide primers were designed to amplify a 343-bp fragment of the flagellin gene of S. typhi. Amplified products were analyzed by agarose gel electrophoresis and Southern blot hybridization by using a 32P-labeled 40-base p...