Erythrocyte glucose-6-phosphate dehydrogenase activity is measured with a centrifugal analyzer by use of a commercial reagent kit and of the reaction glucose-6-phosphate + NADP+ leads to 6-phosphogluconolactone + NADPH. Rate of production of NADPH is measured and related to hemoglobin concentration. Maleimide is added to inhibit further production of NADPH in a secondary reaction by endogenous ...

Pectinolytic enzymes are an important tool for sustainable food production, with a wide range of applications in processing technologies as well the extraction bioactive compounds from pectin-rich raw materials. In present study, we immobilized commercial pectinase preparation onto pellet and thread shaped nylon 6/6 carriers assessed its stability reusability. Five preparations were tested diff...