A microtiter plate assay for quantitation of reduced (GSH) and oxidized (GSSG) glutathione in the rat liver tissue and bile is described. The assay is based on the established enzymatic recycling method and a new thiol-masking reagent, 1-methyl-4-vinyl-pyridinium trifluoromethane sulfonate (M4VP). Samples were first processed by homogenization with (liver) or addition of (bile) sulfosalicylic a...

Reactions of 3-bromo-4-phenylisothiazole-5-carboxamide and 3-bromoisothiazole-5-carboxamide with NaNO2 (4 equiv.), in TFA, at ca. 0 °C gave the carboxylic acid products 99% 95% yields, respectively. The two compounds were fully characterized.

We found that with our assay conditions, TCEP comletely reduced the GSSG that was present within the amples to GSH. Furthermore, the presence of TCEP id not interfere with the labeling of GSH by mBrB nor ith the detection of the glutathione–bimane product. his was true for samples consisting of either purified lutathione or acidified extracts of whole blood. As vidence for this, when samples po...