introduction: the preparation of seasonal influenza virus vaccines and especially its large-scale production requirement after the emergence or reemergence of a pandemic will need an alternative host cell system due to current suboptimal methods and the insufficiency of embryonated chicken eggs needed for producing them. in response to the vital and increasing demand for alternative means for in...

BACKGROUND/AIMS The aim of this study was to evaluate the peritonitis-causing bacteria detected in peritoneal fluid using a blood culture bottle in patients undergoing continuous ambulatory peritoneal dialysis (CAPD). METHODS One-hundred and eleven dialysates from 43 patients suspected of peritonitis related to CAPD were retrospectively evaluated between May 2000 and February 2008. In all cas...

BACKGROUND/AIMS Povidone-iodine (PVP -I) is applied for microbial decontamination of human eyes donated for transplantation. Concentrations and immersion times vary greatly. The effectiveness and toxicity of PVP-I were assessed for different decontamination protocols. METHODS Human donor eyes and corneas were immersed in different concentrations (5-100 mg/ml) of PVP-I for different times (2-3...

A lysis filtration system was used in conjunction with conventional broth culture for 1112 blood cultures. The system, which entailed collection of 5 ml of blood into bottles containing 50 ml isotonic phosphate buffer, Tween 20, and Rhozyme with subsequent filtration using a 0.45 micron Millipore field monitor, was simple and economical to use. Positive results were obtained earlier than those ...

Human lymphocyte culture of two healthy donors was carried out.[3] Later, metaphases were examined for different types of aberrations. For sister chromatid exchange analysis, bromodeoxyuridine (10 μg/ml) was added at the beginning of the culture.[4] Cell cycle kinetics was studied by the BrdU‐Harlequin technique using the replication index (RI) calculated by the standard formula.[5] The Student...

In the advanced stages of AIDS, characterized by severe immunodepression, tuberculosis (TB) may present with a clinical picture of septic shock, due to typical bacteremia. Hematogenic dissemination of mycobacteria is frequent in immunodepressed patients with TB or disseminated mycobacteriosis, leading to increased positivity in detection by automated blood culture. The objective of our study wa...