OBJECTIVES To produce antisera to the 15 recognised reference strains of the Kielstein-Rapp-Gabrielson (KRG) serotyping scheme for Haemophilus parasuis, validate those sera and use them to serotype 46 Australian field isolates of H parasuis. DESIGN Antisera were produced in rabbits and validated by cross-testing with the reference strains and re-testing 15 Australian field isolates of H paras...

Objective: The aim of this study was to estimate genetic variability and phonetic relationships of 50 isolates of P. sojae from Iran. Methods: During 2005–2007, 142 isolates of Phytophthora sojae were collected from soil samples and diseased soybean plants from Lorestan, Mazandaran, Golestan and Ardabil provinces. Races of the isolates were determined. DNA of P. sojae...

Members of the family Enterobacteriaceae are major cause nosocomial infections, including approximately 70% gastrointestinal 60% to urinary tract infections (UTIs), and septicemias. Since 1990, members have emerged as predominant pathogens causing UTIs. In this study, we performed phenotypic testing virulence factors in uropathogens. A total 118 uropathogens belonging were isolated from suspect...

Abstract Background Diagnosis of canine bacterial pneumonia relies on airway lavage to confirm septic, suppurative inflammation, and a positive culture. Considering risks bronchoalveolar fluid (BALF) collection, minimally invasive methods like culture or next generation sequencing blood would be appealing. In dogs with pneumonia, our study aims included (1): determining proportion agreement bet...

Klebsiella pneumoniae is not only a major hospital-acquired pathogen but also an important food-borne pathogen that can cause septicaemia, liver abscesses, and diarrhea in humans. The phenotypic and genotypic characteristics of K. pneumoniae in retail foods have not been thoroughly investigated in China. The objective of this study was to characterize K. pneumoniae isolates through biotyping, s...

Toxoplasma gondii isolates can be grouped into 3 genetic lineages. Type I isolates are considered virulent to outbred mice, whereas Type II and III isolates are not. In the present report, viable T. gondii was isolated for the first time from striped skunk (Mephitis mephitis), Canada goose (Branta canadensis), and black-winged lory (Eos cyanogenia). For the isolation of T. gondii, tissues were ...

The stability of Mycobacterium tuberculosis IS6110 fingerprint patterns and spoligotypes has been assessed by analyzing serial isolates from patients with drug-resistant tuberculosis. Altogether, 165 M. tuberculosis isolates obtained from 56 patients have been analyzed. The time spans between the first and the last or a changed isolate from one patient ranged from 1 to 772 days. Among the 56 pa...

BACKGROUND Mupirocin has been used for the treatment of skin infections and eradication of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA). The increased use of this antibiotic has been accompanied by outbreaks of MRSA that are resistant to mupirocin. OBJECTIVE This study aims to determine the prevalence, genotype and antimicrobial susceptibility of mupirocin-resistant MR...

Sixty clinical isolates of Cryptococcus neoformans var. neoformans were analyzed by random amplification of polymorphic DNA (RAPD) using 12- to 22-mer primers in pairs. Five major profiles, which clearly distinguished between serotypes A (profiles I-III), AD (profile IV), and D (profile V), were identified. Forty-two of 58 serotype A isolates were assigned to profile I, 13 to profile II, and 3 ...

Objective. Despite the provision of local health policies promoting utilization sexual and reproductive (SRH) services adolescents, SRH remains very low little information is known concerning factors which may influence adolescents’ use these services. This study aimed to determine prevalence determinants school-based among secondary school students. Materials Methods. a cross-sectional that u...