Mycobacterium tuberculosis (Mtb) is an aerobic bacterium that persists intracellularly in host macrophages and has evolved diverse mechanisms to combat and survive oxidative stress. Here we show a novel F(420) -dependent anti-oxidant mechanism that protects Mtb against oxidative stress. Inactivation of the fbiC gene in Mtb results in a cofactor F(420) -deficient mutant that is hypersensitive to...

During cofactor F(420) biosynthesis, the enzyme F(420)-γ-glutamyl ligase (FbiB) catalyzes the addition of γ-linked L-glutamate residues to form polyglutamylated F(420) derivatives. In Mycobacterium tuberculosis, Rv3262 (FbiB) consists of two domains: an N-terminal domain from the F(420) ligase superfamily and a C-terminal domain with sequence similarity to nitro-FMN reductase superfamily protei...

There are multiple objectives for the course. Most important among them is to introduce students to a range of basic experimental designs and to provide them with the skills to statistically examine data collected with each type of design. Other important objectives are to provide students with skills: (a) in designing experiments which fit the their substantive questions, (b) in reporting the ...

This study focuses on the semiconductor properties of passive films formed on AISI 420 stainless steel immersed in four nitric acid solutions under open circuit potential (OCP) conditions. For this purpose, the passivation parameters and semiconductor properties of passive films were derived from potentiodynamic polarization and Mott–Schottky analysis, respectively. The OCP plots showed that th...

The tropical storm, floodwater, and the floodplainsediment layer of a 100-year recurrence flood are examined to better understand characteristics of large monsoon floods on medium-sized rivers in northern Thailand. Storms producing large floods in northern Thailand occur early or late in the summer rainy season (May–October). These storms are associated with tropical depressions evolving from t...

In this work, experimental and modelling analyses are performed in order to improve the compression efficiency of a Liquid Piston Gas Compressor (LPGC), which utilizes column water for air compression. Due low cost easy manufacturing, aluminium parallel plates used as heat exchanger inside LPGC absorb thermal energy from compressed hence increase compressor. A comprehensive set numerical gain d...

tau is one of several types of microtubule-associated proteins (maps), responsible for the assembly and stability of microtubule networks that is present only in neurons and predominantly localized in axons which its functions are tightly regulated by phosphorylation. via as yet unknown mechanisms, tau becomes hyperphosphorylated and accompanies with neuronal degeneration, loss of synapses, abe...