Although considerable attention has been directed in the field of gene therapy toward elucidating the mechanism by which a transduced cell could kill a bystander cell, little is known about how bystander cells may affect transduced cells. We hypothesized that bystander cells, particularly if they were capable of gap junctional communication, could protect cells transduced with the herpes simple...

The recommended algorithm for detection and characterization of serum human T-cell lymphotropic virus (HTLV) antibodies calls for first performing a screening enzyme immunoassay (EIA) and then further evaluating repeatedly reactive samples for antibodies recognizing specific HTLV glycoproteins (2, 3). A commercially available Western blot (WB) that includes both native and recombinant HTLV prot...

The aim of this study was to determine whether hemorrhage altered the caspase-3 activity and the ATP levels in rat lung and ileum tissues and determine whether resuscitation with lactated Ringer solution (LR) or whole blood (WB) reversed these changes. Male Sprague-Dawley rats were briefly anesthetized with isoflurane, and their mean arterial blood pressure was reduced from 110 to 40 mmHg by bl...

Testing for Borrelia burgdorferi (BB), the spirochetal agent of Lyme disease, is problematic due to poor sensitivity of commercially available serological tests. We evaluated an in-house Western Blot (WB) prepared with a mixture of two strains of BB, B31 and 297. Sensitivity and specificity of IgG and IgM testing using the two-strain WB was compared to testing with a commercial single-strain WB...

The mechanisms underlying stem cell acquisition of a cardiac phenotype are unresolved. We studied early events during the acquisition of a cardiac phenotype by a cloned adult liver stem cell line (WB F344) in a cardiac microenvironment. WB F344 cells express a priori the transcription factors GATA4 and SRF, connexin 43 in the cell membrane, and myoinositol 1,4,5-triphosphate receptor in the per...

BACKGROUND AND PURPOSE Serodiagnosis of human immunodeficiency virus (HIV) infection typically requires repeatedly reactive positive screening test followed by Western blot (WB) assay. When WB assay result is indeterminate, the results of follow-up WB assay may remain inconclusive. This study evaluated use of enzyme-linked immunosorbent assay (ELISA) and particle agglutination test (PAT) as seq...

В данной работе, посвящённой одной из перикоп Евангелия от Марка, ставится вопрос о том, в каком месте Иисус исцелил прокажённого. Марк не даёт никаких явных указаний на это. Тем менее используемая им лексика (глаголы с приставкой ἐκ(ἐξ-), ἐκβάλλω и ἐξέρχομαι) намекает то, что чудо совершилось закрытом помещении. Это совпадает тем, мы знаем Марка первых чудесах Иисуса: все они происходят помеще...

Current formulations proposed by Eurocode 8 part 3 for the inelastic deformations of existing reinforced concrete members are assessed separately wide beams (WB) and conventional deep (DB). The current approach, based on a large experimental database members, predicts larger ultimate chord rotation but lower ductility WB rather than DB despite similar curvature ductility, due to plastic hinge l...

BACKGROUND Measuring hepatitis C virus (HCV) RNA in serum or plasma may underestimate the true HCV burden. Extracting viral RNA from whole blood (WB) with a cationic surfactant (Catrimox-14) has resulted in HCV RNA concentrations up to 1000-fold higher than from serum or plasma in some studies, but not others. We compared the Catrimox-14 WB assay with a standard serum assay. METHODS Seventy-t...