Cytotoxic action of membrane lipid peroxidation product 4-hydroxynonenal (HNE) is due mainly to its facile reactivity with proteins (Esterbauer, H., Schaur, R. J., and Zollner, H. (1991) Free Radical Biol. Med. 11, 77-80). In the present study, the detailed mechanism of HNE modification of a key enzyme in intermediary metabolism, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), is studied main...

BACKGROUND Glucose-induced protein glycation has been implicated in the progression of diabetic complications and age-related diseases. The anti-glycation potential of polyphenol-rich plant extracts has been shown previously. Bunium Persicum has been demonstrated to possess a high level of polyphenols. The aim of current in vitro study was to determine the possible inhibitory effect of Bunium P...

The variable composition of secondary organic aerosols (SOA) contributes to the large uncertainty for predicting radiative forcing. A better understanding of the reaction mechanisms leading to aerosol formation such as for the photochemical reaction of aqueous pyruvic acid (PA) at λ ≥ 305 nm can contribute to constrain these uncertainties. Herein, the photochemistry of aqueous PA (5-300 mM) con...

In recent years we have been investigating the occurrence, distribution and metabolism of unsaturated amino acids in groundnut plants (Arachi8 hypogaea). Three related amino acids have been identified, namely (i) y-methyleneglutamic acid (y-MGA), (ii) its amide, y-methyleneglutamine (y-MG), and (iii) its decarboxylation product, y-amino-a-methylenebutyric acid (Done & Fowden, 1952; Fowden & Don...

Oxidative stress, an imbalance between the oxidant and antioxidant systems, has been implicated in the pathogenesis of numerous neurodegenerative diseases [1]. Among all the body organs, the brain is particularly vulnerable to oxidative damage because of its high utilization of oxygen, increased levels of polyunsaturated fatty acids, and relatively high levels of redox transition metal ions in ...

Carbonyl products were separated and identified in suspensions of rat liver microsomal fractions and in isolated hepatocytes, after stimulation of lipid peroxidation by incubation with the pro-oxidants CCl4 and ADP-iron. The carbonyl products were allowed to react with 2,4-dinitrophenylhydrazine, and the derivatives were extracted and separated by t.l.c. into three zones of non-polar materials,...

background: the rapid increase in agricultural and industrial development has made heavy metal pollution a serious environmental problem and public health threat; therefore, removal of heavy metals from water is important. the current study prepared dnph@sds@fe3o4 nanoparticles as a novel and effective adsorbent for removal of hg(ii) ions from an aqueous solution. methods: a selective adsorbent...

Background: The rapid increase in agricultural and industrial development has made heavy metal pollution a serious environmental problem and public health threat; therefore, removal of heavy metals from water is important. The current study prepared DNPH@SDS@Fe3O4 nanoparticles as a novel and effective adsorbent for removal of Hg(II) ions from an aqueous solution. Methods: A selective adsorben...