The aim of this study was to form a scaffold-free coculture spheroid model of colonic adenocarcinoma cells (CACs) and normal colonic fibroblasts (NCFs) and to use the spheroids to investigate the role of NCFs in the tumorigenicity of CACs in nude mice. We analysed three-dimensional (3D) scaffold-free coculture spheroids of CACs and NCFs. CAC Matrigel invasion assays and tumorigenicity assays in...

One of the most promising approaches in tissue engineering is the application of 3D scaffolds, which provide cell support and guidance in the initial tissue formation stage. The porosity of the scaffold and internal pore organization influence cell migration and play a major role in its biodegradation dynamics, nutrient diffusion and mechanical stability. In order to control cell migration and ...

Osteoconductive function is remarkably low in bone disease the absence of tissue surrounding grafting site, or if poor condition. Thus, an effective graft terms both osteoconductivity and osteoinductivity required for clinical therapy. Recently, three-dimensional (3D) kagome structure has been shown to be advantageous regeneration due its mechanical properties. In this study, a polycaprolactone...

The aim of this study was to evaluate the behavior of human Dental Pulp Stem Cells (DPSCs), as well as human osteoblasts, when challenged on a Biocoral scaffold, which is a porous natural hydroxyapatite. For this purpose, human DPSCs were seeded onto a three-dimensional (3D) Biocoral scaffold or on flask surface (control). Either normal or rotative (3D) cultures were performed. Scanning electro...

In the central nervous system, numerous acute injuries and neurodegenerative disorders, as well as implanted devices or biomaterials engineered to enhance function result in the same outcome: excess inflammation leads to gliosis, cytotoxicity, and/or formation of a glial scar that collectively exacerbate injury or prevent healthy recovery. With the intent of creating a system to model glial sca...

This study aimed to determine the effect of the 3D bioprinted gelatin-alginate scaffold on the full-thickness skin wound healing on mouse back and to observe the histopathological changes during the wound healing process. Using a murine wound model, full skin thickness excisions were created on the dorsum of 40 mice. Then, each mouse was randomly assigned to either the control group or treatmen...

BACKGROUND Articular cartilage has little capacity for repair in vivo, however, a small number of studies have shown that, in vitro, a damage/repair response can be induced. Recent work by our group has shown that cartilage can respond to single impact load and culture by producing repair cells on the articular surface. The purpose of this study was to identify whether chondrocyte outgrowth int...

Fibroblasts are ubiquitous cells that constitute the stroma of virtually all tissues and play vital roles in homeostasis. The poor innate healing capacity of fibroblastic tissues is attributed to the scarcity of fibroblasts as collagen-producing cells. In this study, we have developed a functional ECM mimicking scaffold that is capable to supply spatial allocation of stem cells as well as ancho...

OBJECTIVE To explore the feasibility of a modified 3D porous small intestinal submucosa (SIS) scaffold seeded with urothelial cells (UC) for surgical reconstruction in a rabbit model. MATERIAL AND METHODS Eighteen New England white male rabbits were divided into three groups and a 0.8 × 1.5 cm(2) section of the anterior urethral mucosa was removed from each animal. Ventral onlay urethroplasty...