qPCR merupakan metode yang akurat untuk mendeteksi ekspresi gen. Analisis gen pengkode faktor transkripsi pada padi saat ini banyak dilakukan. OsAP2, OsERF3 dan OsEREBP2 termasuk kedalam kelompok AP2/ERF berperan penting dalam proses biologis tanaman. Penelitian mengenai analisis Cempo Ireng belum pernah Dalam melakukan menggunakan RT-PCR dibutuhkan primer spesfiik sesuai dengan diinginkan. Pri...

There is an underlying assumption in real-time PCR that the amplification efficiency is equal from the first cycles until a signal can be detected. In this study, we evaluated this assumption by analyzing genes with known gene copy number using real-time PCR comparative gene quantifications. Listeria monocytogenes has six 23S rRNA gene copies and one copy of the hlyA gene. We determined 23S rRN...

A simplified polymerase chain reaction (PCR) assay was developed for fast and easy screening of mycoplasma mastitis in dairy cattle. Species of major mycoplasma strains [Mycoplasma (M.) bovis, M. arginini, M. bovigenitalium, M. californicum, M. bovirhinis, M. alkalescens and M. canadense] in cultured milk samples were detected by this simplified PCR-based method as well as a standard PCR techni...

Arbitrary primed PCR (AP-PCR) analysis was compared with serotyping as a means of high-resolution typing of Pseudomonas aeruginosa. Seventy-four isolates from 3 different hospitals and 18 reference strains were studied. Serotyping provided good index of discrimination, although eleven isolates could not be serotyped. Genomic DNA was amplified with a single 10 nucleotide primer (sequence 5'-AGG ...

Plum pox virus (PPV) is the causal agent of sharka disease of stone fruit trees which is considered to be one of the most serious plant diseases. Sensitive techniques are needed for its diagnosis. Immunosorbent assays based on the use of monoclonal antibodies (1) and different PCR techniques have been described with or without previous immunocapture (2,3). In all instances plant extracts are ne...

Grapevine leafroll disease (GLRD) is one of the most important viral diseases grapevine worldwide. The aim this study was to investigate prevelance associated virus-1 (GLRaV-1), leafroll-associated virus-2 (GLRaV-2), virus-3 (GLRaV-3), virus 4 (GLRaV-4), and virus-9 (GLRaV-9) in Tokat province Turkey. In 2018, leaf samples one-year-old shoots plants, showing virus-like symptoms, were collected ...

Reports describe the association between platelet activation, adhesion, and aggregation, as well polymorphisms in genes encoding membrane glycoproteins with a predisposition to coronary artery disease (CAD). This study investigates associations HPA-1, -3 C807T CAD. A cross-sectional involving 114 women who underwent angiography was performed. The CAD+ group consisted of 63 while CAD- 51 women. ...