A/J mice given urethan (1000 mg/kg) followed by four injections of butylated hydroxytoluene (BHT) (400 mg/kg) developed within 4 mo approximately 40% more lung tumors than mice treated with urethan and given four injections of corn oil. Administration of the metabolic inhibitor piperonyl butoxide prior to BHT injection abolished overall alveolar cell proliferation although it did not completely...

Two newly induced methylcholanthrene sarcomas of C57BL/6 mouse origin were selected for studies of the adoptive immunotherapy of established tumors. The MCA 105 and MCA 106 tumors, used during their first five transplant generations, possessed weakly immunogenic tumor-associated transplantation antigens as revealed by failure to elicit immunity to reject 10(6) tumor cells by tumor growth and ex...

BACKGROUND We previously demonstrated that defective interdomain interaction between N-terminal (0 to 600) and central regions (2000 to 2500) of ryanodine receptor 2 (RyR2) induces Ca2+ leak in failing hearts and that K201 (JTV519) inhibits the Ca2+ leak by correcting the defective interdomain interaction. In the present report, we identified the K201-binding domain and characterized the role o...

Tumor necrosis factor (TNF), a cytokine, and okadaic acid, a tumor promoter, strongly phosphorylated the same proteins, vimentin and heat shock protein 27, although their time courses were different. Human TNF-a at a concentration of 0.6 UMmarkedly stimulated transformation of BALB/3T3 cells initiated with 3-methylcholanthrene. The human TNF-a was about 1000 times more effective than the chemic...

normal, immunodepressed, or immunized recipients, respec tively. A tumor reached 12 mm in diameter more quickly when transplanted into immunized recipients than when transplanted into control animals. Also, the radiation dose that controlled one-half of the treated 12-mm tumors was about 1000 rads higher in immunized than in normal recipients. //; vitro studies showed that lymphoid cells from t...

3-Methylcholanthrene and six of its metabolites were tested for their ability to be metabolized by either hepatic microsomes from Aroclor 1254-pretreated rats or a puri fied hepatic cytochrome P-450-dependent monooxygenase system to products mutagenic to bacterial and mam malian cells. When strain TA98 of Salmonella typhimurium was used to detect mutagens and when microsomes were the source of ...