Studies on plasma and cells exposed to hydroxyl and peroxyl radicals have indicated that there are few inhibitors of protein hydroperoxide formation. We have, however, observed a small variable lag period during bovine serum albumin (BSA) oxidation by 2-2' azo-bis-(2-methyl-propionamidine) HCl (AAPH) generated peroxyl radicals, where no protein hydroperoxide was formed. The addition of free cys...

Objectives. The aim of the present investigation was to study the activity of glucose-6-phosphate dehydrogenase (G6PD) and correlate its activity to protein oxidation markers in type 2 diabetic patients under poor glycemic control. Methods. G6PD activity, protein carbonyl group concentration, and total thiol group content were measured in blood samples of 40 patients with type 2 diabetes mellit...

1.4 respectively. Thus the increase in activity reported by Tomozawa & Wolfenden (1970) on reaction of 6mol of thiol groups/mol of enzyme withp-mercuribenzoate and found again in the present work with 5,5’-dithiobis-(2-nitrobenzoate) results from an increased activation by univalent cations. The effects of the modification of enzyme thiol groups on the response to nucleotide modifiers was also ...

1. A method for selective purification of thiol peptides is described. Thiol groups in a protein are treated with radioactive cystine by disulphide-thiol interchange. The labelled cystine peptides in a digest can then be fractionated for peptide ;maps'. Performic acid oxidation of paper strips containing the radioactive peptides followed by further ionophoresis yields the purified cysteic acid ...

The title compound, [Fe(4)(C(5)H(5)OS)(2)S(CO)(12)], was prepared by the direct reaction of Fe(3)(CO)(12) and 2-furyl-methane-thiol in tetra-hydro-furan. Desulfurization took place readily to form an Fe(4)S(3) cluster. The mol-ecule consists of two similar [(μ-2-C(4)H(3)O-CH(2)S)Fe(2)(CO)(6)] moieties joined to a spiro-type four-coordinate μ(4)-S atom such that this bridging sulfur is tetra-hed...

Thiol-disulfide interconversions play a crucial role in the chemistry of biological systems. They participate in the major systems that control the cellular redox potential and prevent oxidative damage. In addition, thiol-disulfide exchange reactions serve as molecular switches in a growing number of redox-regulated proteins. We developed a differential thiol-trapping technique combined with tw...

Antimicrobial levels of reactive oxygen species (ROS) are produced by the mammalian host defense to kill invading bacteria and limit bacterial colonization. One main in vivo target of ROS is the thiol group of proteins. We have developed a quantitative thiol trapping technique termed OxICAT to identify physiologically important target proteins of hydrogen peroxide (H(2)O(2)) and hypochlorite (N...

in this study an easy, green, efficient and simple approach is reported for the synthesis of some benzothiazole-, benzimidazole- and benzoxazole-2-thiolderivatives. the proposed approach employs the reaction of corresponding aromatic amine with potassium isopropyl xanthate (z11) in the presence of copper sulfate (cuso4) as a catalyst under conventional heating and ultrasonic irradiation. the ad...