Amycolatopsis orientalis DSM 43387, a clinical isolate from submandibular mycetoma tissue, is one of three plasmid-bearing strains of the genus Amycolatopsis. It degrades aromatic compounds such as m-hydroxybenzoate, but does not produce any antibiotics, in contrast to Amycolatopsis orientalis NBRC 12806T. Phylogenetic analysis based on a complete 16S rRNA gene sequence placed the strain in the...

The spectra of radicals formed upon the addition of .OH radicals to the 3 position of substrates for p-hydroxybenzoate hydroxylase (4-hydroxybenzoic acid, 2,4-dihydroxybenzoic acid, and 4-aminobenzoic acid) have been determined using pulse radiolysis combined with the results from high performance liquid chromatography measurements. The 3-hydroxy radical forms of the substrates absorb maximally...

Tetraplatinum cluster complexes bearing hydrophilic anchors, [Pt4(μ-OCOCH3)4(μ-OCOC6H4OH-4)4] (2a), [Pt4(μ-OCOCH3)4(μ-OCOC6H4B(OH)2-4)4] (2b), and [Pt4(μ-OCOCH3)4(μ-OCOC6H4NH2-4)4] (2c), were successfully prepared by a selective substitution reaction of four in-plane acetates of [Pt4(μ-OCOCH3)8] (1) with the corresponding p-substituted benzoic acids. Solid-state structure determination of 2a re...

Ubiquinone is an essential component of the electron transfer system in both prokaryotes and eukaryotes and is synthesized from chorismate and polyprenyl diphosphate by eight steps. p-Hydroxybenzoate (PHB) polyprenyl diphosphate transferase catalyzes the condensation of PHB and polyprenyl diphosphate in ubiquinone biosynthesis. We isolated the gene (designated ppt1) encoding PHB polyprenyl diph...

Berries contain vitamin C and are also a rich source of phytochemicals, especially anthocyanins which occur along with other classes of phenolic compounds, including ellagitannins, flavan-3-ols, procyanidins, flavonols and hydroxybenzoate derivatives. This review examines studies with both human subjects and animals on the absorption of these compounds, and their glucuronide, sulphate and methy...

p-Hydroxyphenylacetate hydroxylase from Acinetobacter baumannii is a two-component system consisting of a NADH-dependent FMN reductase and a monooxygenase (C2) that uses reduced FMN as substrate. The crystal structures of C2 in the ligand-free and substrate-bound forms reveal a preorganized pocket that binds reduced FMN without large conformational changes. The Phe-266 side chain swings out to ...

As part of a study on the assimilation of organic compounds by autotrophic microorganisms, enrichment cultures were set up for thiobacilli. A facultatively autotrophic Thiobacillus was isolated in pure culture and grew heterotrophically on many organic compounds including p-hydroxybenzoate (PHBz). Since degradation of aromatic compounds is carried out by a limited range of bacterial genera, the...

The antimicrobial properties of cis-6-hexadecenoic acid (C16:1Δ6), a component of the innate human metabolome, were studied and its application to cosmetic products was investigated in detail. A variety of the resident and transient microbial flora of the skin, oral cavity, and intestine was applied to an investigation of the antimicrobial activity of C16:1Δ6. C16:1Δ6 showed selective antibacte...

BACKGROUND Very few data are available from the literature on whether nonatopic subjects affected by persistent rhinitis may show the appearance of objective symptoms of rhinitis after the ingestion of food additives such as tartrazine (E102), erythrosine (E127), monosodium benzoate (E211), p-hydroxybenzoate (E218), sodium metabisulphite (E223), and monosodium glutamate (E620). It is still uncl...

BACKGROUND: Self-assembling amphiphilic block copolymers with incorporated, biologically relevant functionalities have received limited attention, partly due to the fact that biomolecules are not robust enough to synthetic manipulation, do not lend themselves readily to systematic studies due to their complexity and leach rather quickly from a vesicle or membrane when physically incorporated. S...