The extracellular pH (pi I,.) in many solid tumors is often lower than in normal tissues. Cells may survive conditions of acid pi 1, because antiports in their membrane exchange Na* for 11+,or 11CX)., for Cl~, and thus regulate the intracellular pH (pH,). We have therefore assessed the effects of drugs which interfere with regulation of pi I; on survival of Chinese hamster ovary and human bladd...

Background: Some respiratory diseases may induce alveolar hypoxia thereby hypoxic pulmonary vasoconstriction (HPV). However, the mechanisms of this physiologic phenomenon are not fully understood. This study was the first to investigate the role of anion exchanger in sustained HPV.Methods: Experiments were performed in the isolated perfused rabbit lung. After preparation, the lungs were divided...

INTRODUCTION The aim of this work was to examine the influence of quercetin on chronic intoxication of rats with ammonium fluoride. A 1:1 mixture of quercetin 8,5' disulfonic acid sodium salts (Na2QDSA): NaQSA-5' and NaQSA-8 (Figure 1), synthesized at the Department of Inorganic Chemistry, Technical University of Rzeszów, was used. Pharmacological investigations have demonstrated the diverse ac...

The patch-clamp technique was used to determine the ionic conductances activated by ATP in murine colonic smooth muscle cells. Extracellular ATP, UTP, and 2-methylthioadenosine 5'-triphosphate (2-MeS-ATP) increased outward currents in cells with amphotericin B-perforated patches. ATP (0.5-1 mM) did not affect whole cell currents of cells dialyzed with solutions containing ethylene glycol-bis(β-...

We have investigated the volume-activated transport of organic solutes in flounder erythrocytes. Osmotic swelling of cells suspended in a Na(+)-free medium led to increased membrane transport of taurine, glucose, and uridine. For each compound there was a significant lag period (1-2 min at 10 degrees C) between cell swelling and activation of the flux. The volume-activated fluxes of each of the...

A highly efficient and simple method for the preparation of α,α´-bis(arylidene)cycloalkanones under solvent-free conditions has been developed. The crossed-aldol condensation of cycloalkanones (1 eq.) with arylaldehydes (2 eq.) in the presence of 2.5 mol% of ionic liquid 1,3-disulfonic acid imidazolium hydrogen sulfate {[Dsim]HSO4} afforded the title compounds in high yields and short reaction ...

We examined the voltage-dependent block of Caactivated Cl channels by anthacene-9-carboxylic acid (A9C), diphenylamine-2-carboxylic acid (DPC), 4,4*-diisothiocyanostilbene-2,2*-disulfonic acid (DIDS), and niflumic acid (NFA) in excised inside-out and outside-out patches from Xenopus oocytes. The fraction of the voltage field (d) experienced by the blocking drug was determined from the voltage d...