mahaleb (prunus mahalab l.) is used as a rootstock for cherries in iran and many other countries. in this research, rapd markers were used to determine the genetic diversity among eight genotypes of mahaleb evaluated in two separate research grardens in karaj, iran. these genotypes are used as mother plants for seed production. totally 40 random primers were examined for pcr reactions on the te...

Southern hybridization analysis of the MASP1 gene using an intron-specific probe detected a single band. An exon-specific probe detected several bands. PCR of genomic DNA using several exon-specific primer sets of MASP1 produced short and long products. Sequence of the shorter products corresponded to the processed pseudogene of MASP1. By fluorescence in situ hybridization, this pseudogene (MAS...

Cytoplasmic membrane-associated DNA (cmDNA) is a species of DNA that attaches to the plasma membrane and has physical and chemical properties that differ from those of bulk chromosomal and mitochondrial DNAs. Here, we used deep sequencing to analyze cmDNA and showed that satellite DNAs consisting of both of simple (CCATT)(N) repeats from the pericentromere regions of the chromosomes and 171-bp ...

One virulent phage specific to Bacillus Subtilis was isolated from soil cultivated with liquorices root in Matruh governorate, Cairo, Egypt. The B. subtilis phage isolate produced 0.13 cm diameter circular plaques; turbid and without central zone. In vitro determination of thermal inactivation point (TIP), dilution end point (DEP) and longevity in vitro (LIV) were found to be 68°C/10 min, 10 an...

Antigenic variants of Leptospira interrogans serovars copenhageni and hebdomadis were examined by bacterial restriction endonuclease DNA analysis with EcoRI, XhoI, SalI, BstEII, and HindIII as the digesting enzymes. The antigenic variants were stable cloned strains which had been cultivated in media containing homologous immune serum. One of the strains examined has been reported elsewhere (R. ...

This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not ...

The shape of the chromosomal DNA of the sulfur-dependent archaebacterium Sulfolobus acidocaldarius was analyzed by the pulsed-field gel electrophoresis(PFGE). S.acidocaldarius DNA digested with Notl showed two DNA bands at around 1.0 Mbp and 2.1 Mbp. Notl-linking clones were isolated from the library of S.acidocaldarius chromosomal DNA. It contained two Notl sites. Both 1.0 and 2.1 Mbp DNA band...

Santa Rosa and Frontier are the major Japanese plum (Prunus salicina Lindl.) cultivars grown throughout world. The present investigation was performed to understand genetic relatedness among in vitro propagated using PCR based molecular markers. For study, three arbitrary markers viz. RAPD (Random amplified Polymorphic DNA), ISSR (Inter-Simple Sequence Repeats) SCoT (Start Codon Targeted) were ...

Two-alternative forced-choice procedures were used to measure the detectability of bright and dark bars at various locations across luminance patterns that produced Mach bands. Detection performance was significantly affected by both dark and bright Mach bands: poor detection performance was observed at locations near, but not in, the Mach bands; relatively good detection performance at locatio...

The Catskill Mountains of New York State are an important source of wild-collected American ginseng (Panax quinquefolium) and, increasingly, of woods-cultivated ginseng. The objective of this study was to assess genetic diversity among 9 different wild ginseng populations in and adjacent to the Catskill Mountain region of New York State and to compare these to wild populations from other states...