MCF-7 cells have been shown to contain estrogen receptor in several cell fractions following homogenization: nuclei, microsomes, and cytosol. The amount of 17 beta-estradiol-binding capacity found in each cellular compartment depended on the inclusion of detergent in homogenization buffers and on the use of 0.25 M sucrose in the nuclear washes. 17 beta-Estradiol receptor (E2R) associated with n...

background: in previous studies, the neuroprotective effect of 17&beta-estradiol; in diffuse traumatic brain injury has been shown. this study used ici 182,780, a non-selective estrogen receptor antagonist, to test the hypothesis that the neuroprotective effect of 17&beta-estradiol; in traumatic brain injury is mediated by the estrogen receptors. methods: the ovariectomized rats were divided in...

Removal of estradiol-17 beta from plasma by immunoaffinity chromatography on anti-estradiol antibodies coupled to Sepharose was investigated and compared with the use of charcoal adsorbent. Both methods completely removed added [3H]estradiol from pooled male plasma. In contrast to the adsorbent treatment, which caused marked changes in the biochemical composition of the plasma, immunoaffinity c...

The aim of the presented study was to compare the effect of 17-beta-estradiol on the hydrolytic activity of plasma membrane calcium pump (PMCA) purified from excitable (rat cortical synaptosomes) and non-excitable (human erythrocytes) cells. Both types of cell membranes contained different composition of the PMCA isoforms. To elucidate if the hormone action could depend on structure of PMCA pro...

The oxidative metabolism of estradiol was studied in normal men and women by a radiometric procedure that provides information on the totality of the biotransformations concerned. The release of 3H into body water from estradiol labeled with 3H in the 17 alpha, 16 alpha, and C-2 positions permits measurement of the rate and extent of 17 beta-ol oxidation and of the competing hydroxylations at C...

We have studied the effects of endogenous and exogenous estrogen on atherosclerotic lesions in apolipoprotein E-deficient mice. Female mice ovariectomized (OVX) at weaning displayed increases (P < 0.01) in fatty streak lesions in the proximal aorta and aortic sinus compared with female mice with intact ovarian function. These differences between the OVX and sham controls were apparent in both c...

Background: The presence of estrogen receptor beta and aromatase in the germ cell has highlighted the physiological role of the traditionally female hormone, estrogen, in spermatogenesis. Estrogen receptor alpha knockouts and aromatase knockouts have further accentuated the role of estrogen in germ cell maturation. To delineate effects of high intratesticular estradiol in the seminiferous epith...

Membrane-associated binding sites with high affinity and specificity for estrogens have been identified in calf uterine microsomes. The binding of 17 beta-[3H]estradiol is specific and saturable at low hormone concentration (2 nM) of high affinity (Kd = 0.5 nM) and sensitive to trypsin and other proteolytic enzymes. Binding of [3H]estradiol to membranes is inhibited by low concentrations of unl...

BACKGROUND AND OBJECTIVES: Nitric oxide (NO) is a potent vasodilator and estrogen-mediated vasodilation that increases NO production. The association of the vascular endothelium, gender and vasodilation induced by estrogen is due to the activation of two estrogen receptors, alpha (ERa) and beta (ERb). The aim of this study was to compare NO production stimulating receptors ERa and ERb with the ...