Ochradenus Delile is a small genus of 6-9 species within family Resedaceae. In Egypt, it is only represented by the gynodioecious Ochradenus baccatus Delile, the most widespread species across the entire range of the genus. Within Egypt, field populations and herbarium specimens of O.baccatus showed vast morphological and gender diversity within and between different populations even in the sam...

Random amplified polymorphic DNA (RAPD) technique is a simple and reliable method to detect DNA polymorphism. Several factors can affect the amplification profiles, thereby causing false bands and non-reproducibility of assay. In this study, we analyzed the effect of changing the concentration of primer, magnesium chloride, template DNA and Taq DNA polymerase with the objective of determining t...

Genetic diversity of two wild Kalibaus, Labeo calbasu populations and one hatchery stock was studied using random amplified polymorphic DNA (RAPD) method. The three 10–mer random primers (OPA01, OPB02 and OPC03) yielded a total of 26 reproducible and consistently scorable RAPD bands of which 15 (57.69%) were considered as polymorphic (P95) indicating a high level of genetic variation in all the...

Currently, there are three published HLA-B*15:02 screening methods for prevention of carbamazepine-induced severe drug reactions in Asian populations. To analyze available HLA-B*15:02 screening methods, we compared four screening methods, including a multiplex PCR method, a nested PCR method, a LAMP method and our new in-house PCR-dot blot hybridization method. These methods were reviewed regar...

Restriction fragment length polymorphisms (RFLP) of the intergenic spacer region (IGS) of rDNA and random amplified polymorphic DNA (RAPD) markers were used to survey genetic variability among 181 isolates of Sclerotinia homoeocarpa from Ontario and 10 isolates from Japan. RAPD and IGS-RFLP analyses revealed polymorphisms within and between populations of S. homoeocarpa, distinguishing 151 geno...

Ten Synthetic Hexaploid Wheats (SHWs) (2n = 6x = 42, AABBDD) were analyzed using 7 Randomly Amplified Polymorphic DNA (RAPD) primers to investigate the potential genetic diversity resided within the D-genome of common wheat. Total 190 fragments were amplified in which 141 were polymorphic showing 74 % of polymorphism. Polymorphism Information Content (PIC), Genetic Similarity (GS) Coefficient, ...

The aim of this study was to investigate the genetic diversity and markers associated with salinity tolerance in three genotypes wheat created for salt by plant breeding program, as well two Iraqi varieties using random amplified polymorphic cDNA (RAPD-PCR) eight primers were used. results RAPD marker revealed that there are variations several particular segments various sizes between selected ...

genetic diversity is the basis success of improving the quality and quantity of crops. recognizing of genetic diversity and genetic potential of each plant species in plant breeding is necessary. in this study genetic diversity of 30 lentil samples belonging to collection of the college of agriculture of krage were evaluated using rapd molecular markers. rapd products were scored for presence (...

rapd markers were employed to determine the diversity among 29 genotypes and cultivars of sour and duke cherry as well as a mahaleb tree genotype as an outgroup. a number of 100 random primers were examined for pcr reactions on the template dnas extracted from leaves, of which 17 primers produced polymorphic bands. these 17 selected rapd primers produced 233 bands, among which, 214 were polymor...

Twenty four apricot genotypes with different geographic origins, maintained in the germplasm collections of Central Institute of Temperate Horticulture, Srinagar, India, were studied by means of RAPD markers. The aim of the study was to determine the genetic relationship amongst genetypes from different eco-geographical regions. Fourty three decamer primers were used in the study. All of them w...