نتایج جستجو برای: 1 dna vaccine

تعداد نتایج: 3216706  

Journal: :BMC Infectious Diseases 2007
Jianjun Chen Fenghua Zhang Fang Fang Haiyan Chang Ze Chen

BACKGROUND Maternal antibody is the major form of protection against disease in early life; however, its presence interferes with active immunization of offspring. In order to overcome the immunosuppression caused by maternal antibody, several immune strategies were explored in this paper using mouse model and influenza vaccines. RESULTS The results showed that: i) when the offspring were imm...

Journal: :Immunological reviews 2004
Marie J Estcourt Andrew J McMichael Tomás Hanke

Development of a vaccine against human immunodeficiency virus type 1 (HIV-1) is the main hope for controlling the acquired immunodeficiency syndrome pandemic. An ideal HIV vaccine should induce neutralizing antibodies, CD4+ helper T cells, and CD8+ cytotoxic T cells. While the induction of broadly neutralizing antibodies remains a highly challenging goal, there are a number of technologies capa...

Journal: :Hong Kong medical journal = Xianggang yi xue za zhi 2009
K Y Yuen P C Y Woo S K P Lau

1. When different forms of SARS coronavirus (SARS-CoV) spike protein-based vaccines for generation of a neutralising antibody response to SARS-CoV were injected into a mouse model, all the mice immunised with intramuscular tPA-optimised 800 DNA vaccine boosted with intraperitoneal recombinant spike polypeptide generated by Escherichia coli and intramuscular CTLA4Hinge SARS800 DNA vaccine booste...

Journal: :Journal of virology 2003
Danilo R Casimiro Ling Chen Tong-Ming Fu Robert K Evans Michael J Caulfield Mary-Ellen Davies Aimin Tang Minchun Chen Lingyi Huang Virginia Harris Daniel C Freed Keith A Wilson Sheri Dubey De-Min Zhu Denise Nawrocki Henryk Mach Robert Troutman Lynne Isopi Donna Williams William Hurni Zheng Xu Jeffrey G Smith Su Wang Xu Liu Liming Guan Romnie Long Wendy Trigona Gwendolyn J Heidecker Helen C Perry Natasha Persaud Timothy J Toner Qin Su Xiaoping Liang Rima Youil Michael Chastain Andrew J Bett David B Volkin Emilio A Emini John W Shiver

Cellular immune responses, particularly those associated with CD3(+) CD8(+) cytotoxic T lymphocytes (CTL), play a primary role in controlling viral infection, including persistent infection with human immunodeficiency virus type 1 (HIV-1). Accordingly, recent HIV-1 vaccine research efforts have focused on establishing the optimal means of eliciting such antiviral CTL immune responses. We evalua...

Journal: :iranian journal of virology 0
k borhani department of virology, school of medical sciences, tarbiat modares university, tehran, iran t bamdad department of virology, school of medical sciences, tarbiat modares university, tehran, iran m ajorloo department of virology, school of medical sciences, tarbiat modares university, tehran, iran shr mozhgani human rabies vaccine laboratory, production and research complex pasteur, institute of iran, tehran, iran n miandehi viral vaccines production department, production and research complex pasteur, institute of iran, tehran, iran a moradi-joshaghan viral vaccines production department, production and research complex pasteur, institute of iran, tehran, iran

background and aims: the aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcdna3.1(+) in bsr cell line. this construct might be used for a potential dna vaccine. materials and methods: glycoprotein gene was synthesized and cloned into pbluescript vector and then sub cloned into eukaryotic expression vector (pcdna3.1(+)). after verifica...

2012
Guohong Ge Shixia Wang Yaping Han Chunhua Zhang Shan Lu Zuhu Huang

Although the use of recombinant hepatitis B virus surface (HBsAg) protein vaccine has successfully reduced global hepatitis B infection, there are still a number of vaccine recipients who do not develop detectable antibody responses. Various novel vaccination approaches, including DNA vaccines, have been used to further improve the coverage of vaccine protection. Our previous studies demonstrat...

2010
Sirika Pillay Enid G Shephard Ann E Meyers Anna-Lise Williamson Edward P Rybicki

Several approaches have been explored to eradicate HIV; however, a multigene vaccine appears to be the best option, given their proven potential to elicit broad, effective responses in animal models. The Pr55Gag protein is an excellent vaccine candidate in its own right, given that it can assemble into large, enveloped, virus-like particles (VLPs) which are highly immunogenic, and can moreover ...

2010
Eric S. Rosenberg Barney S. Graham Ellen S. Chan Ronald J. Bosch Vicki Stocker Janine Maenza Martin Markowitz Susan Little Paul E. Sax Ann C. Collier Gary Nabel Suzanne Saindon Theresa Flynn Daniel Kuritzkes Dan H. Barouch

BACKGROUND An effective therapeutic vaccine that could augment immune control of HIV-1 replication may abrogate or delay the need for antiretroviral therapy. AIDS Clinical Trials Group (ACTG) A5187 was a phase I/II, randomized, placebo-controlled, double-blinded trial to evaluate the safety and immunogenicity of an HIV-1 DNA vaccine (VRC-HVDNA 009-00-VP) in subjects treated with antiretroviral ...

Journal: :iranian biomedical journal 0
زهرا گرزین zahra gorzin علی اکبر گرزین ali akbar gorzin علیجان تبرایی alijan tabarraei ناصر بهنامپور naser behnampour شیوا ایرنی shiva irani امیر قائمی amir ghaemi

backgrounds: most of the hepatitis c virus (hcv) infections elicit poor immune responses and 75% to 85% of cases become chronic therefore, the development of an effective vaccine against hcv is of paramount importance. in this study, we aimed to evaluate co-administration of hcv non-structural protein 2 and il-12 dna vaccines in c57bl/6 mice. methods: a plasmid encoding full-length hcv ns2 prot...

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